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Introduction of Some New Technologies (1)

— 10X GENOMICS CHROMIUM™

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November 19, 2018

10X GENOMICS CHROMIUM™

10x Genomics Chromium Controller – High-throughput automated barcoding and library construction for powerful new RNA and DNA sequencing applications. The Chromium Controller is powered by 10x GemCode Technology and enables the encapsulation in a single run of up to 80,000+ individual cells or from as little as 1 ng of HMW gDNA into 100,000s to 1,000,000s of uniquely barcoded picoliter droplets for downstream genomic analysis with Chromium Software Suite. Chromium technical applications have revolutionized single-cell RNA-seq and 10x Linked-Read technology now provides long-range information from short-read sequencing data.

Technical process:

10X GENOMICS CHROMIUM™

Key technical principles:

10X GENOMICS CHROMIUM™

Application:

  • Genome&Exome
  • Single Cell RNA-Seq
  • Single Cell V(D)J + 5′Gene Expression
  • De Novo Assembly

Reference:

  1. Yan K S, Gevaert O, Zheng G X Y, et al. Intestinal Enteroendocrine Lineage Cells Possess Homeostatic and Injury-Inducible Stem Cell Activity[J]. Cell Stem Cell, 2017, 21(1):78-90. e6.
  2. Dixit A, Pamas O, Li B, et al. Perturb-Seq: Dissecting Molecular Circuits with Scalable Single Cell RNA Profiling of Pooled Genetic Screens[J]. Cell.2016 Dec 15; 167(7): 1853-1866. e17.
  3. Yan K S, Janda C Y, Chang J, et al. Non-equivalence of Wnt and R-spondin ligands during Lgr5+ intestinal stem-cell self-renewal[J]. Nature, 2017, 545(7653): 238-242.
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