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New Sequencing Technique Can Register Modification Level Change in tRNA December 19/2018

A new high-throughput sequencing technique created by scientists at the University of Chicago will directly analyses transfer RNA (tRNA) to give a clear picture of microbial communities’ reactions to environment changes. The technique, outlined in Nature Communications, will uncover how naturally occurring microbiomes respond to real-world conditions and diets.......

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Introduction of Some Newly Launched Sequencers (1) December 14/2018

Oct. 27, 2018--MGI, a subsidiary of BGI Group, introduced its newest model of genetic sequencer, MGISEQ-T7, which vastly improves speed, throughput and flexibility, at the 13th International Conference on Genomics (ICG-13) in Shenzhen. The most powerful MGI sequencer to date, MGISEQ-T7 is built with innovative quadruple flow-cell staging that allows simultaneous but independent operation of 1 to 4 flow cells in a single run. The MGI proprietary technology used in T7 delivers higher accuracy and improves efficiency through upgrades to the flow cell, fluid, and biochemical and optical system. A supercomputer for the life science industry, MGISEQ-T7 takes the production capacity of the sequencer to a new level with daily output of data up to 6Tb.......

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Introduction of Some Newly Launched Sequencers (2) December 10/2018

Jan. 8, 2018-- Illumina, Inc. (NASDAQ: ILMN) announced today the launch of the iSeq™ 100 Sequencing System. The new, next-generation sequencing (NGS) system delivers exceptional data accuracy, at a low capital cost, making Illumina technology available to virtually any lab. The combination of the company’s proven sequencing by synthesis (SBS) chemistry, along with the complementary metal-oxide-semiconductor (CMOS) detection technology, represents an entirely different configuration that delivers highly accurate data at substantially lower capital costs. The iSeq 100 is the first system with this architecture and has a U.S. list price of $19,900.......

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Introduction of Some Newly Launched Sequencers (3) December 4/2018

The Ion S5/S5 XL next-generation sequencing system enables a simple targeted sequencing workflow for your lab at an affordable price, with reliable performance and industry-leading speed. The Ion S5/S5 XL System leverages the speed of semiconductor sequencing with impressive on-board computing power, to enable the production of high quality sequencing data in as little as 2.5 hours and enable you to go from DNA library to data in as little as 24 hours with only 45 minutes of total hands-on time on the Ion Chef™ System and the Ion S5/S5 XL System. The Ion S5/S5 XL System is simple to use with cartridge-based reagents and offers superior scalability and flexibility to support a broad range of high throughput sequencing applications, from microbial genomes and gene panels to exomes and transcriptomes.......

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The Birth of CRISPR-Edited Twins November 29/2018

He Jiankui, the Chinese scientist who stunned the world by announcing the birth of two CRISPR-edited twins this week. He claimed to have turned off a gene called CCR5 to offer total protection against HIV, as well as smallpox and cholera. On the international gene-editing summit in Hong Kong, he said he was “proud” to have edited the genes of the two twin girls.......

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New Uses for Single Cell RNA Sequencing Data November 22/2018

In the era of personalized medicine, scientists are using new genetic and genomic insights to help them determine the best treatment for a given patient. It is now possible to look at what one single cell is doing at any given time with a technique called single-cell RNA sequencing (scRNA-seq). This method looks at the amount of messenger RNAs (mRNAs) in a cell and compares those to other cells to look for differences in gene expression.......

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Introduction of Some New Technologies (1) November 19/2018

10x Genomics Chromium Controller – High-throughput automated barcoding and library construction for powerful new RNA and DNA sequencing applications. The Chromium Controller is powered by 10x GemCode Technology and enables the encapsulation in a single run of up to 80,000+ individual cells or from as little as 1 ng of HMW gDNA into 100,000s to 1,000,000s of uniquely barcoded picoliter droplets for downstream genomic analysis with Chromium Software Suite. Chromium technical applications have revolutionized single-cell RNA-seq and 10x Linked-Read technology now provides long-range information from short-read sequencing data.......

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Introduction of Some New Technologies (2) November 16/2018

ATAC-seq, or Assay for Transposase-Accessible Chromatin coupled with NGS, is a technique to locate accessible chromatin regions. The method is a fast and sensitive alternative to DNase-seq (DNase I hypersensitive sites sequencing) or MNase-seq (micrococcal nuclease sensitive sites sequencing).By using this service, we can detect genome-wide profiles of open and accessible regions of chromatin that are indicative of active regulatory regions. The eukaryotic genome is highly packaged to fit into the very limited nuclear space. As a result, access to genomic information is tightly regulated based on cellular state. What regions of the genome are accessible reveals a great deal about the state of the cell.......

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Introduction of Some New Technologies (3) November 13/2018

Hi-C method is based on Chromosome Conformation Capture, in that chromatin is crosslinked with formaldehyde, then digested, and re-ligated in such a way that only DNA fragments that are covalently linked together form ligation products. The ligation products contain the information of not only where they originated from in the genomic sequence but also where they reside, physically, in the 3D organization of the genome. In Hi-C, a biotin-labeled nucleotide is incorporated at the ligation junction, making it possible to enrich for chimeric DNA ligation junctions when modifying the DNA molecules for deep sequencing. The compatibility of Hi-C with next generation sequencing platforms makes it possible to detect chromatin interactions on an unprecedented scale. This advance gives Hi-C the power to both explore the chromatin biophysics as well as the implications of chromatin structure in the biological functions of the nucleus. A massively parallel survey of chromatin interaction provides the previously missing dimension of spatial context to other genomic studies. This spatial context will provide a new perspective to studies of chromatin and its role in genome regulation in normal conditions and in disease.......

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Introduction of Some New Technologies (4) November 9/2018

DNA methylation strongly affects chromatin structure and the regulation of gene expression. For many years, bisulfite sequencing PCR (BSP) has served as the “gold standard” for measuring DNA methylation. BSP has high reliability and accuracy, and can accurately determine the methylation status of each CpG site in the fragment.......

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