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The Bagworm Genome Reveals A Unique Fibroin Gene That Provides High Tensile Strength Apri 29/2019

Arthropod silk is known as a versatile tool, and its variability makes it an attractive biomaterial. Eumeta variegata is a bagworm moth (Lepidoptera, Psychidae) that uses silk throughout all life stages. Notably, the bagworm-specific uses of silk include larval development in a bag coated with silk and plant materials and the use of silk attachments to hang pupae. An understanding at the molecular level of bagworm silk, which enables such unique purposes, is an opportunity to expand the possibilities for artificial biomaterial design.......

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The Faster and Cheaper High-Throughput RNA Sequencing Apri 19/2019

Genome-wide gene expression analyses by RNA sequencing (RNA-seq) have quickly become a standard in molecular biology because of the widespread availability of high throughput sequencing technologies. Nonetheless, RNA sequencing is still expensive and time-consuming, because it first requires the costly preparation of an entire genomic library—the DNA pool generated from the RNA of cells—while the data itself are also difficult to analyze. All this makes RNA sequencing difficult to run, rendering its adoption not as widespread as it could be.......

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New “Shredder” CRISPR Technique Can Delete Large Stretches of DNA Apri 12/2019

Recently, a new article entitled "Introducing a Spectrum of Long-Range Genomic Deletions in Human Embryonic Stem Cells Using Type I CRISPR-Cas" was published in Molecular Cell journal, revealing a new gene editing tool which goes beyond the usual mechanisms of CRISPR,and that acts more like a shredder, able to target and delete much longer expanses of DNA than the current Cas9 tool is capable of.......

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The Effects of Poverty on Human Genes Apri 5/2019

A new Northwestern University study challenges prevailing understandings of genes that long-term poverty can be “embedded” across the genome.......

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New CRISPR-Chip Can Finds DNA Mutations without Amplification March 25/2019

A team of engineers at the UC Berkeley and the Keck Graduate Institute (KGI) of The Claremont colleges combined CRISPR with electronic transistors made from graphene to create a new hand-held device that can detect specific genetic mutations in a matter of minutes, and can be used to quickly diagnose genetic disorders and diseases or determine the accuracy of gene-editing techniques.......

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High-Throughput Screening And Classification of Chemicals And Their Effects on Neuronal Gene Expression Using RASL-seq March 14/2019

We previously used RNA-seq to identify chemicals whose effects on neuronal gene expression mimicked transcriptional signatures of autism, aging, and neurodegeneration. However, this approach was costly and time consuming, which limited our study to testing a single chemical concentration on mixed sex cortical neuron cultures. Here, we adapted a targeted transcriptomic method (RASL-seq, similar to TempO-seq) to interrogate changes in expression of a set of 56 signature genes in response to a library of 350 chemicals and chemical mixtures at four concentrations in male and female mouse neuronal cultures.......

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Crowdfunded Whole-Genome Sequencing of The Celebrity Cat Lil BUB Identifies Causal Mutations for Her Osteopetrosis And Polydactyly February 26/2019

Rare diseases and their underlying molecular causes are often poorly studied, posing challenges for patient diagnosis and prognosis. The development of next-generation sequencing and its decreasing costs promises to alleviate such issues by supplying personal genomic information at a moderate price. Here, we used crowdfunding as an alternative funding source to sequence the genome of Lil BUB, a celebrity cat affected by rare disease phenotypes characterized by supernumerary digits, osteopetrosis and dwarfism, all phenotypic traits that also occur in human patients.......

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New Generation of cDNA Sequencing–Oxford Nanopore Introduces New cDNA Sequencing Kits February 4/2019

Oxford Nanopore has launched its two new ‘109’ cDNA Kits for its real-time, scalable sequencing technology. The new kits provide high throughput while generating complete sequences of full-length cDNA strands, with a low input option of just 1ng PolyA+ RNA.......

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China Clones Gene-Edited Monkeys January 25/2019

Scientists at the Institute of Neuroscience (ION) in China have reportedly used gene-editing technology to knocked out BMAL1, a core circadian regulatory transcription factor, using gene editing at the embryo stage in macaque monkeys, one of which was then cloned to produce five virtually identical specimens. This prototype could create populations of genetically identical monkeys to allow scientists to better study the mechanisms of complex human disorders.......

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Chromosome-Level Assembly of The Water Buffalo Genome Surpasses Human And Goat Genomes In Sequence Contiguity January 16/2019

Rapid innovation in sequencing technologies and improvement in assembly algorithms have enabled the creation of highly contiguous mammalian genomes. Here we report a chromosome-level assembly of the water buffalo (Bubalus bubalis) genome using single-molecule sequencing and chromatin conformation capture data.......

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