CD-Genomics provides Immune Repertoire Sequencing Service to amplify and sequence the immune repertoire with Next Generation Sequencing (NGS).
Immune repertoire is the sum total of functionally diverse T and B cells in a body at any given moment. Immune Repertoire Sequencing is to amplify the complimentary determining region (CDR) of B-cell receptor (BCR) or T-cell receptor (TCR) using multiple-PCR or 5' RACE methods, followed by high-throughput sequencing. Sequencing the expressed T- and B-cell receptor genes in the immune repertoire allows for an accurate evaluation of a person's health status and risks for different diseases.
BCRs and TCRs are similarly organized. Each receptor is composed of two distinct subunit chains (BCR: light chain and heavy chain, TCR: α chain and β chain). The antigen binding surface is formed by the variable region of each chain, which is encoded by recombined V, J, and D (BCR heavy and TCRβ) gene segments. (Jorg J.A. Calis et al. Trends in Immunology, 2014.12)
Our expert technicians will sequence your samples at our secure, state-of-the-art laboratory. They'll use our proprietary multiplex PCR with highly optimized primer sets and deep sequencing techniques that exclusively target T-cell and B-cell receptor genes. With the Immune Repertoire Sequencing Service the following panel are available:
CD Genomics basic data analysis includes: