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MassARRAY SNP Genotyping

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CD Genomics offers a rapid and accurate custom SNP validation on the MassARRAY MALDI–TOF instrument provided by Agena Bioscience. Our MassARRAY SNP Genotyping system combines mass spectrometry, sensitive and robust chemistry, and advanced data analysis software to meet your SNP genotyping needs.

The Introduction of MassARRAY SNP Genotyping

MassARRAY SNP Genotyping amplifies the DNA sequence across the SNP site by PCR, and then uses a single extension primer to amplify PCR products, ensuring that the primer extends only one base. The extended product was analyzed by TOF (time of flight) and the SNP was classified according to the molecular weight difference of base, and the allele frequency of the SNP site could also be calculated. MassARRAY SNP genotyping can carry out multiple SNP typing in one reaction. This technique is accurate and has a price advantage when analyzing dozens or hundreds of SNPs.
With different detection and software modules, the MassARRAY® iPLEX GOLD technology can realize the research or application of SNP genotyping, gene expression research, copy number variation, gene methylation analysis, pathogen typing and prenatal diagnosis on one platform. MassARRAY SNP Genotyping has a very good application prospect in the fields of biological science, medicine, agricultural research and so on, it has provided excellent solutions for scientists all over the world.

Applications

  • Genotyping and mutation detection
  • Ultrasensitive detection
  • Methylation analysis

Advantages of MassARRAY SNP Genotyping

  • Accurate and Automated: Nucleic acid detection by mass spectrometry enables readout by molecular mass, and using state-of-the-art technologies for automated, non-contact dispensing of reagents
  • Scalable and Flexible: We offer a variety of options for SNP, sample and assay throughput.
  • High Multiplex Capability: Multiplex up to 30 SNP loci in a single pool.
  • Fast Assay Design: A new assay can be designed within a few hours. In addition, a semi-automated workflow and unmodified oligos provide rapid turnaround time
  • Cost Effective: Multiplex analysis reduces per-sample cost, and scalable throughput optimizes batch and resource requirements.

MassARRAY SNP Genotyping Workflow
The procedures for our MassARRAY SNP Genotyping is illustrated below.

Service Specification

Sample and Submission requirements
  • Sample type: genomic DNA
  • DNA concentration:20-50 ng/µL, DNA volume: ≥ 15 µL OD260/280 = 1.7~2.0 without degradation or RNA contamination
Chip Printing
  • Sequenom MassARRAY iPLEX Gold Platform
Bioinformatics Analysis
  • Genotypes are determined through ratio analysis of the MALDI-TOF mass spectrometer
  • Quality control and detection of oligonucleotides
  • SNP typing and SNP allele frequency calculation
  • Detection and analysis of somatic mutation, methylation quantitative analysis

Deliverables

  • The original sequencing data
  • Experimental results
  • Data analysis report

Our service includes customized SNP panels design, oligo manufacture and pool balancing, iPLEX chemistry running, analysis of genotyping and delivery of genotype report in Excel format. Our workflows can be designed to accommodate both small and large assay requirements. If you have additional requirements or questions, please feel free to contact us.

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CONTACT CD GENOMICS

45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-275-3058
Fax: 1-631-614-7828
Email: info@cd-genomics.com