Sequenom MassARRAY iPLEX Gold

iPLEX® Gold is a leading technology for SNP Genotyping. The MassARRAY system is widely used for fine mapping and validation of GWAS studies linkage studies, as well as routine genetic testing of SNP panels of interest. MassARRAY SNP Genotyping combines the benefits of a simple and accurate primer extension chemistry with state of the art MALDI-TOF mass spectrometry to quickly and cost effectively characterize genotypes with the highest levels of reproducibility. The iPLEX Gold assay used in hundreds of publications to routinely design assays at a multiplexing level of up to 40-plex, offering a high level of flexibility.

iPLEX detection technology facilitates routine assay designation at a multiplex level of 36-plex, offering a high degree of flexibility and cost-efficiency for each genotype. The iPLEX Single Base Extension (SBE) chemical methodology features an elevated level of precision and reproducibility (>99% call rate and >99.7% accuracy in validated tests), in addition to a low allelic frequency detection of as little as 5%. This enables the achievement of stringent call thresholds and superior performance at a high level of multiplexing.

The MassARRAY® System adeptly streamlines the detection process for 10 to 400 Single Nucleotide Polymorphisms (SNPs) per sample, thereby enabling the analysis of a spectrum of samples, ranging in quantity from a handful to several hundreds per day. It possesses stand-alone functionality but also the capacity to interact and integrate seamlessly with Next-Generation Sequencing (NGS) platforms. This intricate compatibility system significantly expedites the procedure of genetic analysis. Introduced in the dawn of the new millennium in 2000, the MassARRAY System has been earnestly and widely employed in an array of genomic applications, each with its own complexities and demands. These applications encompass fine mapping, the validation process of Genome-Wide Association Studies, intricate linkage analyses, as well as the conventional genetic analyses of SNPs and incipient somatic mutations.

Highlights:

Flexibility:

• Free selection of SNP sites of interest
• Freedom to choose the number and position of samples on a single chip
• Freedom to pair samples and SNP sites on a single chip

Accuracy:

• Direct detection of the molecular weight of the target, with an accuracy exceeding 99.7%
• Mass spectrometry technology can flexibly detect PCR experiment failures or the presence of tri-allelic genes

High throughput:

• Up to 384 samples can be multiplexed in a single iPLEX GOLD experiment on a single chip
• Up to 40 reactions can be achieved per reaction well
• Up to one hundred thousand genotype analyses can be performed per day

Cost-effectiveness:

• No need for fluorescent labeling, significantly reducing costs
• Even in low throughput scenarios, the cost of each genotype analysis remains low

Service Content

• Customers provide genomic DNA or biological samples and specify the SNP sites to be detected.
• Primer design, including PCR amplification primers and single-base extension primers.
• Design of multiplex reactions.
• PCR amplification, SAP treatment, single-base extension.
• Preparation of spotted arrays for detection.
• MALDI-TOF mass spectrometry detection.
• Data analysis and provision of genotype reports.

Applications:

• Whole-genome association study validation
• Individual SNP genotype analysis
• Haplotype analysis
• SNP gene site frequency analysis
• Epigenetic analysis
• Somatic mutation analysis