Whole Genome Bisulfite Sequencing Q&A

  • General Questions

  • What factors does Bisulfite-Seq need to consider before starting a project?
  • Can Bisulfite studies be conducted on species lacking reference genomes?
  • If we construct our own libraries, can we proceed with on-board sequencing? Additionally, how can we assess the library's quality and ensure the sequencing results are reliable?
  • What are the plant DNA methylation sequencing methods?
  • Is it possible to test plants for DNA methylation of target genes?
  • Can PCR amplification of plant target gene methylation be achieved effectively through the design of specific primers for the target gene?
  • Whole genome methylation sequencing of plants can avoid the problem of designing primers for methylation of target genes?
  • What is the minimum DNA requirement for WGBS library construction when dealing with a genome size of ≤1.5G (where m≥2μg)?
  • What is the efficiency of Bisulfite conversion?
  • How to characterize methylation-supportive reads in Bisulfite-Seq?
  • Can Bisulfite-Seq count the methylation frequency?
  • WGBS and WGS can be jointly analyzed, what is the main analysis?
  • Why does hydroxymethylation exhibit less data in the methylation and hydroxymethylation heatmap?
  • What are the requirements for RRBS library construction?
  • Recommended methods for DNA methylation research in plants when the budget is limited?
  • What are the necessary requirements for Whole Genome Bisulfite Sequencing (WGBS) in a species?
  • What data volume is recommended for Whole-Genome Bisulfite Sequencing (WGBS)? Are biological repeats necessary?
  • WGBS project mapping rate is relatively low compared to other projects?
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