CD genomics can help you analyze genetic variation efficiently to meet diverse research needs. By utilizing Affymetrix and Illumina array platforms, we support high-throughput and multiplex processing to meet diverse research needs, delivering high-quality data at a low per-sample cost.
The Introduction to SNP Microarray
Powered by state-of-the-art variant detection and SNP genotyping technologies, CD genomics provides various genotyping services, delivering high-quality data at a low per-sample cost. Microarray is a valuable tool to enable researchers to explore genetic variants such as single nucleotide polymorphisms (SNPs) and large structural changes, from discovery applications to routine screening.
Affymetrix and Illumina genotyping microarrays both provide trusted performance with respect to precision medicine initiatives, clinical and translational research, pharmacology, consumer screening, and agricultural applications. Based on the species and the selection of SNPs, multiple genotyping strategies are available.
Table 1. Our available automated SNP genotyping platforms.
|GeneTitan instrument together with Axiom array plates||
|GeneChip Scanner 3000 7G System together with Affymetrix GeneChip||
|Illumina iScan system together with Illumina BeadChips||
Advantages of SNP Microarray
- Custom, flexible, and scalable;
- High call rates (> 99%) and high accuracy;
- Cost-effectiveness and high-throughput;
- Identifies SNPs in a targeted or whole genome scale;
- In addition to SNPs, other genetic differences, such as copy number variations, can be measured
- Applied in biomarker discovery and validation, clinical testing, GWAS, pharmacogenomics, forensics, and breed discrimination.
SNP Microarray Workflow
The general workflow for SNP Microarray is outlined below. We have three well-recognized genotyping platforms (Table 1) for genome-wide or targeted identification of SNPs and copy number variations in a high-throughput and affordable manner. Our highly experienced expert team executes quality management, following every procedure to ensure confident and unbiased results.
We provide customized bioinformatics analyses including:
Our Full Service covers the complete process, from quality control of genomic DNA to the determination of genotypes. Besides, CD Genomics can also help create your own custom microarray which can be tailored to meet specific project requirements. If you have additional requirements or questions, please feel free to contact us.
1.The introduction of Illumina and Affymetrix genotyping platforms.
Table 1. Affymetrix and Illumina genotyping solutions.
Affymetrix genotyping solutions
|The Affymetrix SNP microarray applies a combination of photolithography and combinatorial chemistry to directly synthesize oligonucleotides on a glass surface. Affymetrix arrays can achieve very high density to accommodate millions of probes on a single chip.|
Illumina genotyping solutions
|Illumina's unique BeadArray technology is based on 3-micron silica beads that self-assemble in micro-wells on planar silica slides. Each bead is covered with hundreds of thousands of copies of a specific oligonucleotide, which acts as the capture sequence. Accuracy and reliability of the assays are usually above 99.5%.|
2.How to choose the most suitable genotyping platform for my specific needs?
The choice of genotyping strategies depends on the species, the purpose and the number of SNPs per sample, as well as the number of samples. For different cases, we recommend different genotyping technologies.
- For genome-wide SNP genotyping, we recommend Illumina BeadChips, Affymetrix GeneChips or NGS.
- For gene-wide SNP genotyping, such as fine mapping or haplotyping of candidate regions, we recommend NGS or array-based technologies.
- For the analysis of individual SNPs, we recommend Real-time PCR assays, Sanger Sequencing, Illumina BeadChips, Affymetrix technology or NGS.
3.What arrays are available at CD Genomics?
At CD Genomics, we have both ready-made and custom arrays for your SNP genotyping projects.
Table 2. Our available arrays for SNP genotyping.