CD Genomics has extensive experience in providing support for the selection and design of microsatellite markers for a wide range of plant and animal species. We offer a full service which includes assistance in designing and ordering fluorescently labeled primer pairs for the microsatellite markers of your choice.
The PCR reaction is performed with fluorescent dye-labeled primers, then the PCR fragments can be analyzed on a capillary DNA sequencing machine (ABI 3730xl DNA Analyzer), and the data is analyzed using AB GeneMapper software. By taking advantage of multiplexing by fragment size and dye color, and the ability of automated fluorescent genetic analyzer machines to autoload 16 samples at a time from 96-well or even 384-well microtitre plates, high throughput marker analysis experiments can be designed. This service has been applied to a wide variety of species and can accommodate any species for which microsatellite markers are available.
The vast amount of data emerging for thousands of microsatellite markers across organisms makes microsatellite genotyping a widely accepted tool for:
- Linkage analysis of co-segregation
- Diagnosis and identification of human diseases
- Forensic identification and relatedness testing
- Population studies
- Microsatellite instability
Microsatellite Genotyping Procedures
Why Choose CD Genomics?