Single-Cell RNA Sequencing Q&A

  • General Questions

  • What are the characteristics of 10X Genomics single cell labeled RNA?
  • Can I do circRNA sequencing by 10X Genomics single cell sequencing?
  • How do I obtain Marker genes in single cell transcriptome sequencing?
  • What single-cell transcriptome sequencing projects have you been involved in?
  • What is the difference between 5'-end and 3'-end capture for single cell sequencing library construction?
  • Why is the 5' end capture method usually used for VDJ?
  • What association analysis can be done for single-cell transcriptome sequencing and single-cell ATAC sequencing?
  • How to choose the depth of single-cell RNA sequencing?
  • How many cells can be measured at a time by single-cell transcriptome sequencing?
  • How efficient is the single-cell capture of single-cell transcriptome? Will there be multiple cells captured?
  • What is the typical amount of data obtained from a single cell?
  • What kinds of analysis can be used from scRNA sequencing?
  • What preferences exist in single-cell RNA sequencing
  • What is important to note about the reverse transcription step in scRNA sequencing?
  • What to look for in amplification process steps in scRNA sequencing?
  • What are the quality control checkpoints in scRNA sequencing?
  • What is the problem if there are few spike-in RNA sequences?
  • What is the problem if the scRNA sequencing results find high mitochondrial RNA?
  • What is the problem if a high percentage of ribosomal RNA is found in scRNA sequencing result?
  • Sample Preparation

  • What should I do if I tend to form clumps during the preparation of single cell suspensions?
  • How do I prepare samples for single cell transcriptome sequencing?
  • Why is the cell viability required after single-cell suspension preparation so high?
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