New Generation of cDNA Sequencing--Oxford Nanopore Introduces New cDNA Sequencing Kits

February 4, 2019

Oxford Nanopore has launched its two new ‘109’ cDNA Kits for its real-time, scalable sequencing technology. The new kits provide high throughput while generating complete sequences of full-length cDNA strands, with a low input option of just 1ng PolyA+ RNA.

The two new kits now available are Direct cDNA (SQK-DCS109) and PCR-cDNA (SQK-PCS109) Sequencing Kits. In combination with upgrades to the Oxford Nanopore sequencing platforms and software, nanopore cDNA sequencing delivers effective enrichment for full-length transcripts (PCR-cDNA). In the case of the Direct cDNA kit, PCR is not necessary — removing bias, while the cDNA- PCR kit enables high throughput from low RNA input (just 1 ng PolyA+ RNA) and provides the option of starting from total RNA.

The new cDNA kits are available to use on all Oxford Nanopore devices, letting all types of user generate very high yields of transcriptomic data for high-resolution novel isoform discovery, identification of low copy number transcripts and de novo sequencing of entire transcriptomes to high coverage.

The new cDNA sequencing method enables users to sequence whole transcriptomes to high coverage on a single MinION flow cell (10-15 million reads x full length transcripts), with substantially higher throughput capabilities on PromethION (more than 60 million reads x full length transcripts per flow cell), or smaller, rapid assays on Flongle. This facilitates new biological insights across a diverse range of applications.

Delving deeper into the transcriptome lets researchers better understand how an organism’s DNA is interpreted at any one moment. This could better inform treatment in healthcare and provide a quick method of identifying viruses.

Both kits are now available to buy on Oxford Nanopore’s site.