Metagenome Sequencing Q&A

  • General Questions

  • What are the differences between metagenome and microbial diversity?
  • How to choose between metagenome sequencing or diversity analysis?
  • What is the difference between metagenome and metatranscriptome?
  • How to perform metagenome assembly?
  • Why perform metagenome assembly?
  • What factors are associated with the effectiveness of metagenome assembly?
  • In metagenome assembly, why can't all sample data be combined together for assembly?
  • In the assembly process, is it that the common high-abundance genes can be assembled, but the individual-specific low-abundance genes cannot be assembled?
  • What is the recommended amount of sequencing for different samples?
  • Can metagenome sequencing detect information about microorganisms such as bacteria, fungi, protozoa, plankton and viruses?
  • For metagenomic projects, how to exclude host contamination?
  • Can metagenome sequencing be performed if there is a large amount of host contamination and there is no reference sequence of the host genome?
  • Which genes are annotated by KEGG?
  • Do I need to have biological replicates for Meta-analysis?
  • Sample Preparation

  • What are the considerations for metagenomic soil sample preparation?
  • What are the considerations for metagenomic manure sample preparation?
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