MeRIP Sequencing Q&A

  • General Questions

  • Can meRIP-seq study m6A modifications of RNA?
  • Can m6A be analyzed in association with circ RNA?
  • Is it true that m6A-seq does not need to be greater than 3 samples per group, and is it okay to do m6A without duplicates and only once per sample?
  • Why are the genes of chloroplast and mitochondria compared in MeRIP data?
  • Are MeRIP and RIP similar?
  • How to design MeRIP-seq experiments?
  • Do I need to interrupt after RNA enrichment in MeRIP-seq?
  • How to elute the RNA from m6A antibody if my RNA volume is low
  • How to extract the RNA after IP?
  • What is your MeRIP-seq library building and sequencing process?
  • What MeRIP-seq analysis services do you offer?
  • What are the subsequent experimental validations of MeRIP-seq?
  • Is it necessary to do MeRIP-seq in addition to normal transcriptome sequencing?
  • Why is it necessary to do both Input and IP samples for MeRIP-seq?
  • Is there any species restriction to perform MeRIP-seq?
  • Sample Preparation

  • What are the sample delivery requirements for MeRIP-seq?
  • Is it better to use RNA directly or cDNA for MeRIP-seq?
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