CD Genomics-the genomics service company
Search
Search CD Genomics
Home / Sequencing / Genomics Sequencing / Whole Genome Sequencing

Whole Genome Sequencing

Bookmark and Share
  • Details
  • FAQ
  • Case Study

CD Genomics has been providing the accurate and affordable whole genome sequencing service for couple of years. We combine both Illumina (short reads) and PacBio (long reads) platforms to achieve whole genome de novo assemblies and re-sequencing for virus, microbes, plants, animals and humans.

The Introduction of Whole Genome Sequencing

Whole genome sequencing represents the determination of the complete DNA sequence of an organism’s genome at a single time, which entails chromosomal DNA as well as DNA contained in mitochondria and chloroplasts. Whole genome sequencing provides a powerful tool for both de novo sequencing and re-sequencing. De novo sequencing refers to sequencing of a novel genome without reference sequence available. The coverage quality of de novo assembly depends on the size and continuity of the contigs. De novo sequencing generates the first genome map for a species, thus providing a valuable reference sequence for re-sequencing. Oxford Nanopore and PacBio sequencing systems enable a faster and more accurate characterization of any species at the nucleotide level.

Whole genome resequencing can identify DNA biomarkers such as single nucleotide polymorphism (SNPs), insertions and deletions (indels), structure variations (SVs), copy number variations (CNVs) and other genetic changes of the sequenced species with high accuracy. It also provides an unprecedented opportunity for characterizing the polymorphic variants in a population, which comprehensively unravels the underlying mechanisms of species origin, development, growth, and evolution. What’s more, whole genome re-sequencing is an indispensable part of genome-wide association study (GWAS), where common genetic variants in different individuals are assessed to determine if a variant is associated with a particular phenotype. GWAS can be broadly used in food safety, agriculture, and pharmacy, especially the personalized medicine.

Advantages of Whole Genome Sequencing

  • Single base-pair resolution
  • De novo sequencing and genome-wide mutation characterization
  • Population evolution and phylogenetic studies
  • Disease research, drug discovery and development, and personalized medicine

Whole Genome Sequencing Workflow

CD Genomics combines both Illumina HiSeq and PacBio systems to provide a fast and accurate whole genome sequencing and bioinformatics analysis for any species. Our highly experienced expert team executes quality management, following every procedure to ensure confident and unbiased results. The general workflow for whole genome sequencing is outlined below.

Service Specifications

Sample requirements and preparation
  • DNA amount ≥ 2 μg, DNA concentration ≥ 20 ng/μl, OD260/280=1.8~2.0.
  • All DNA samples are validated for purity and quantity.
Sequencing
  • Illumina HiSeq X Ten, PacBio SMRT, or Oxford Nanopore.
  • Depth of coverage ≥ 100x.
  • More than 80% of bases with a ≥Q30 quality score.
  • Both paired-end library and mate-pair library can be constructed in the library preparation step.
Bioinformatics Analysis
We provide customized bioinformatics analysis including:
  • Raw data quality control
  • Statistics of sequencing depth and coverage
  • De novo assembly, reference genome mapping
  • SNP/InDel/SV/CNV calling
  • Annotation and statistics
  • Pathway enrichment analysis
  • Population genetics analysis
  • More data mining upon your request

Analysis pipeline

CD Genomics provides full whole genome sequencing service package including sample standardization, library construction, deep sequencing, raw data quality control, genome assembly, and bioinformatics analysis. We can tailor this pipeline to your research interest. If you have additional requirements or questions, please feel free to contact us.

Related Services
Whole Exome Sequencing

Uncover disease-causing mutations in the exome

Targeted Region Sequencing

information of the selected targets

Quote Request
Name:
Phone:
Organization:
* Email:
* Services & Products of Interest:
Project Description:
* Verification Code:
Please input "genomics" as verification code.
Feedback

Accept problems, make progress together, work hard to succeed

READ MORE
Featured Publications

Keep abreast of the latest application status

READ MORE
CONTACT CD GENOMICS

45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-275-3058
Fax: 1-631-614-7828
Email: info@cd-genomics.com