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SNaPshot Multiplex System for SNP Genotyping

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CD Genomics offers SNP genotyping using simple and affordable SNaPshot Multiplex System to allow efficient and quick results.

The Introduction of SNaPshot

The SNaPshot Multiplex System is a primer extension-based method for genotyping known SNP positions through the automated DNA analyzer invented by Applied Biosystems. Through its multiplexing capability, up to 10 SNPs can be analyzed in a single reaction by using unlabeled, user-defined primers. SNPs can be interrogated regardless of their position on the chromosome or separation from a neighboring SNP locus. The Multiplex Ready Reaction Mix helps ensure robust, reproducible analyses of multiplexed samples. SNaPshot Multiplex System can screen and confirm SNPs, and detect minor sequence variations.

Advantages of SNaPshot

  • High accuracy
  • Saving time and money
  • Require little optimization
  • High throughput level
  • Consistence and easy to use
  • Multiplex capability allows low cost
  • Sensitive allele-frequency detection
  • Automated analysis

SNaPshot Workflow

Firstly, the primers were used to amplify the target SNPs fragment, and Exo I and the Shrimp Alkaline Phosphatase (SAP) were added to the amplification products to digest the primer and the remaining dNTPs. Then, using the purified products as template, the PCR reaction was performed using the sequencing enzyme, four fluorescent labeling ddNTP and the 5 '- terminal extension primers close to the SNP site. The primers extended one base only. After the ABI sequencer, the corresponding SNP loci were determined according to the position and color of the peak. According to the color of the peak, we can know the type of the base, and identify the gene sequence of the sample. It is usually used for analysis of 3 to 30 SNP sites. A typical SNaPshot Multiplex System for SNP genotyping workflow is shown as follow:

Service Specifications

Sample requirements and preparation
  • DNA amount ≥ 1 µg and concentration ≥ 20 ng/µl, OD260/280 = 1.8~2.0
  • All DNA samples are validated for purity and quantity
Sequencing
  • ABI PRISM 3700 DNA Analyzer
Bioinformatics Analysis
  • ABI Genescan™ software package
  • Disease research: linkage analysis and association studies
  • Human identification: forensic and paternity testing
  • Molecular diagnostic research: Fragile X, cystic fibrosis (CF) and loss of heterozygosity (LOH) assays
  • Livestock breeding: animal parentage testing and animal genotyping
  • Agricultural and microbial typing: amplified fragment length polymorphism (AFLP®) technique

Deliverables

  • The original sequencing data
  • Experimental results
  • Data analysis report
  • Details in SNaPshot for your writing (customization)

With professional bioinformatics capability, CD Genomics offers high-quality SNP genotyping services for you. If you have any questions, please feel free to contact us.

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