Pan-Cancer Panel Sequencing

Pan-Cancer Panel Sequencing

Description of Pan-Cancer Panel

Pan-Cancer Panel is a predesigned panel for targeted resequencing assay utilizing amplicon sequencing technology by Illumina system, designed to detect somatic variants and assessing tumor mutational burden (TMB) in tumor samples. The panel covers a total of 516 genes associated with major solid tumor types and maintains >96% correlation with whole exome datasets. CD Genomics utilizes targeted NGS sequencing technology to provide more efficient and accurate targeting of specific genes or mutations, and even to detect low frequency variations in cancer-associated genes. Targeted NGS sequencing facilitates sequencing of a large number of genes and samples in a single and cost-effective assay.

CD Genomics provides a pan-cancer panel sequencing and bioinformatics analysis for your cancer related researches.

Gene List of Pan-Cancer Panel

ABL1 ABL2 ACVR1 ACVR1B AGO2 AKT1 AKT2 AKT3 ALK ALOX12B
AMER1 ANKRD11 APC AR ARAF ARFRP1 ARID1A ARID1B ARID2 ARID5B
ASXL1 ASXL2 ATM ATR ATRX AURKA AURKB AXIN1 AXIN2 AXL
B2M BABAM1 BAP1 BARD1 BBC3 BCL10 BCL2 BCL2L1 BCL2L11 BCL2L2
BCL6 BCOR BCORL1 BIRC3 BLM BMPR1A BRAF BRCA1 BRCA2 BRD4
BRIP1 BTG1 BTK CALR CARD11 CARM1 CASP8 CBFB CBL CCND1

(Check the Pan-Cancer Panel Sequencing Gene List for more genes.)

Highlights of Pan-cancer Panel Sequencing Service

  • Target-enrichment sequencing by Illumina provides extremely high depth (average depth > 1000x) with lower costs.
  • High coverage uniformity ensures the sequencing data more efficiency.
  • SNVs, indels, and even low frequency variations can be detected.
  • Strict quality control throughout the pipeline workflow to ensure the accuracy and repeatability of the sequencing.
  • Fast turnaround time.

Applications of Pan-cancer Panel Sequencing

  • Cancer related mechanism studies
  • Biomarker discovery
  • Therapeutic target discovery
  • Drug target discovery
  • Discover rare mutations and detect low frequency mutations

Service Specifications

Service Specifications

Sample Requirements

  • Sample types for genome DNA from tissues, whole blood, circulating cells, cultured cells, and formalin-fixed paraffin-embedded (FFPE) tissues.
  • Recommended amount: 2μg.
  • Minimum amount: 200ng.
  • Collection: DNA samples are stored in TE buffer or equivalent. Blood is collected by routine blood collection kits.
Service Specifications

Sequencing

  • Illumina PE150
  • Enrichment Method: Amplicon sequencing
  • More than 80% of bases with a ≥ Q30 quality score
  • Recommended sequencing depth: ≥ 1000x
Service Specifications

Bioinformatics Analysis

We provide customized bioinformatics analysis including:

  • Read alignment
  • Variant calling/Annotation
  • Tumor mutation burden (TMB) analysis

Gene Panel Workflow

CD Genomics provides the accurate and cost-effective pan-cancer panel sequencing and bioinformatics analysis. Our professional expert team executes quality management, following every procedure to ensure confident and unbiased results. The general workflow for pan-cancer panel sequencing is outlined below.

Gene Panel Workflow

For more information about the pan-cancer panel or need other amplification requirements, please contact us.

References:

  1. Zhen W X, et al. Assessment of tumor mutation burden calculation from gene panel sequencing data. oncotarget and therapy, 2019. 12, 3401—3409.
  2. Miller, E M, et al. Development and validation of a targeted next generation DNA sequencing panel outperforming whole exome sequencing for the identification of clinically relevant genetic variants. Oncotarget, 2017. 8(60),102033-102045.
* For Research Use Only. Not for use in diagnostic procedures.

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