Pathogens Capture Sequencing

Description

Rapid and accurate retrieval of whole genome sequences of human pathogens from disease vectors or animal reservoirs will enable fine-resolution studies of pathogen epidemiological and evolutionary dynamics. However, next generation sequencing technologies have not yet been fully harnessed for the study of vector-borne and zoonotic pathogens, due to the difficulty of obtaining high-quality pathogen sequence data directly with a high ratio of host to pathogen DNA.

CD Genomics' pathogens capture is designed to enrich genomes of different pathogens and their subtypes, which could be further analyzed through next generation sequencing platforms. This panel platform is cost-effective and effectively help researchers to identify different types of pathogens, analyze mutations on the pathogens and explore pathogenic mechanism, providing a new insight into medical research.

Highlights of Pathogens Capture Sequencing Service

  • 500-50000 kinds of microorganisms captured all at once including bacteria, viruses and disease-causing parasites.
  • Targeted sequencing technology by illumina system provides ultra-deep sequencing to target specific genomic regions.
  • Strict quality control throughout the pipeline workflow to ensure the accuracy and repeatability of the sequencing.
  • Fast turnaround time.
  • Provide customized pathogen genome capture service.

Applications of Pathogens Capture Sequencing

  • Study the mechanism of pathogen infection
  • Resistance-related mutation detection
  • Genotyping
  • Help make treatment strategies

Service Specifications

Service Specifications

Sample Requirements

  • Sample types for genomic DNA from whole blood.
  • Recommended amount: 2μg
  • Minimum amount: 1μg
  • Collection: DNA samples are stored in TE buffer or equivalent. Blood is collected by routine blood collection kits.
Service Specifications

Sequencing

  • Illumina PE150
  • Enrichment Method: Hybridization capture
  • More than 80% of bases with a ≥ Q30 quality score
  • Recommended sequencing depth: ≥ 500x
Service Specifications

Bioinformatics Analysis

We provide customized bioinformatics analysis including:

  • Read alignment
  • Variant calling/Annotation
  • Genotyping

Gene Panel Workflow

CD Genomics provides the accurate and cost-effective pathogens capture sequencing and bioinformatics analysis. Our professional expert team executes quality management, following every procedure to ensure confident and unbiased results. The general workflow for pathogens capture sequencing panel is outlined below.

Gene Panel Workflow

For more information about the pathogens capture or need other amplification requirements, please contact us.

Reference:

  1. Clark S A, et al. Targeted DNA enrichment and whole genome sequencing of Neisseria meningitidis directly from clinical specimens[J]. International Journal of Medical Microbiology, 2018. 308.
* For Research Use Only. Not for use in diagnostic procedures.

Online Inquiry
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  • 45-1 Ramsey Road, Shirley, NY 11967, USA
  • 1-631-275-3058 (USA)
           44-208-144-6005 (Europe)
  • 1-631-614-7828
  • info@cd-genomics.com
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