HBV/HPV/EBV Capture Sequencing

Description

As infectious agents, viruses are a cause of significant health problem world-wide, especially in the developing nations. Infection of viruses could lead to various human diseases such as influenza, AIDS and cancers. With the development of technology, use of next generation sequencing to study globally relevant viral infections have revolutionized our understanding of the within host and between host evolution of these viruses.

CD Genomics' HBV/HPV/EBV capture sequencing is designed for target enrichment of different viruses and their subtypes, which is necessary to detect low abundance virus populations, in next generation sequencing (NGS) applications on Illumina ® platforms. This panel platform could effectively help researchers to identify different types or subtypes of viruses, analyze mutations on the viruses and help make treatment strategies, providing a new insight into medical research especially in cancer area.

Gene List of HBV/HPV/EBV Capture Sequencing

Virus Type Product Description Suitable Scope
HBV Able to detect HVB subtype A, B, C, D, E, F, G, H and other known subtypes HBV infected liver cancer or other HBV infection causing disease study
HPV Including 6, 11, 16, 18, 31, 33, 35, 39, 45, 52, 58, 59, 66, 68, 69, 82 and other known subtypes Cervical cancer and other HPV integration-driven cancer
EBV Whole EBV genome capture Nasopharynx Cancer, Lymphoma and other EBV infection-caused cancer

Highlights of HBV/HPV/EBV Capture Sequencing Service

  • Targeted sequencing technology by illumina system provides ultra-deep sequencing to target specific genomic regions.
  • Strict quality control throughout the pipeline workflow to ensure the accuracy and repeatability of the sequencing.
  • Fast turnaround time.

Applications of HBV/HPV/EBV Capture Sequencing

  • Study the mechanism of viral infection
  • Viruses genotyping
  • Help make treatment strategies

Service Specifications

Service Specifications

Sample Requirements

  • Sample types for genomic DNA from cervical exfoliated cells, urogenital tract secretion samples and other Infection parts.
  • Recommended amount: 1μg
  • Minimum amount: 200ng
  • Collection: DNA samples are stored in TE buffer or equivalent.
Service Specifications

Sequencing

  • Illumina PE150
  • Enrichment Method: Hybridization capture
  • More than 80% of bases with a ≥ Q30 quality score
  • Recommended sequencing depth: ≥500x
Service Specifications

Bioinformatics Analysis

We provide customized bioinformatics analysis including:

  • Read alignment
  • Variant calling/Annotation
  • Viruses genotyping

Gene Panel Workflow

CD Genomics provides the accurate and cost-effective HBV/HPV/EBV capture sequencing and bioinformatics analysis. Our professional expert team executes quality management, following every procedure to ensure confident and unbiased results. The general workflow for virus genome capture sequencing is outlined below.

Gene Panel Workflow

For more information about the HBV/HPV/EBV capture sequencing or need other amplification requirements, please contact us.

* For Research Use Only. Not for use in diagnostic procedures.

Online Inquiry
  • Please input "genomics" as verification code.

  • 45-1 Ramsey Road, Shirley, NY 11967, USA
  • 1-631-275-3058 (USA)
           44-208-144-6005 (Europe)
  • 1-631-614-7828
  • info@cd-genomics.com
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