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Solid Tumor Whole Exome Sequencing

Solid Tumor Whole Exome Sequencing

Introduction

Exome sequencing mainly refers to the capture and enrichment of exomes and surrounding regions of DNA, followed by the use of next generation sequencing (NGS) to obtain their sequence information, and finally combined with bioinformatics analysis to reveal the association of variants (SNPs/ Indels/SVs/CNVs) with diseases. Whole exome sequencing (WES) is widely applied in the identification of the genetic causes of diseases, especially in tumor cases. Sequencing the DNA coding region of tumor cells not only detects genetic mutations, but also provides information about any developmental delay. According to the study, 85% of known disease mutations are located in protein coding regions (exons), so we can detect a more concentrated target associated with solid cancers by WES.

CD Genomics specializes in disease genomics such as hereditary genetic disorders and cancer. And our rapid NGS workflow will maximize efficient and cost-effective whole exome sequencing service and bioinformatics support for our clients in solid tumors research. Our solid tumor sequencing analysis services are designed to provide you with a molecular landscape of cancer and a new profile of gene mutations.

Highlights of Our Solid Tumor WES Service

We Can Help You With

Service Specifications

Service Specifications

Sample Requirements

  • Sample types: genomic DNA, formalin-fixed paraffin-embedded (FFPE) tissues
  • Recommended amount: 2 μg
  • Minimum amount: 200 ng
  • Collection: DNA samples (stored in TE buffer or equivalent), FFPE samples (recommended cryopreservation). Please make sure that the DNA is not degraded.
Service Specifications

Sequencing

  • Illumina PE150
  • More than 80% of bases with a ≥ Q30 quality score
  • Recommended sequencing depth: ≥100×
Service Specifications

Bioinformatics Analysis

  • Turnaround time: within 6 weeks
  • Primary analysis: read alignment, SNP/Indel/CNV annotation
  • Secondary analysis: statistical and pathway analysis, NMF mutation characterization, driver gene prediction
  • Tertiary analysis: susceptibility gene screening, variants analysis, structure analysis, tumor purity and ploidy analysis
  • Custom bioinformatics analysis

Workflow of Our Service

We are committed to using cutting-edge molecular profiling techniques to advance the genetic basis and developmental mechanisms of genetic diseases, cancer, and other important diseases. Our rapid NGS workflow can be adapted and optimized to your needs.

Workflow of Our Service

We also offer custom solid tumor panel services that target a wide range of common solid tumors and cancer hotspots, suitable for exploring the molecular pathogenesis of solid tumors and developing targeted drugs. For more information, please contact us.

* For Research Use Only. Not for use in diagnostic procedures.

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