Glioma Gene Panel Sequencing

Glioma Gene Panel Sequencing

Description of Gliomas

Gliomas are the most common type of primary brain tumors that start in the brain or spinal cord. Gliomas are composed predominantly of astrocytomas, oligodendrogliomas, ependymomas, and mixtures of various glial cells such as oligoastrocytomas or glioblastoma with an oligodendroglial component. On average, 5 out of every 100,000 people suffer from malignant glioma per year. Although malignant glioma is relatively uncommon, it has an extremely high recurrence and mortality rates. People with malignant gliomas may have the following symptoms: headaches, nausea and vomiting, epilepsy, focal neurologic deficits, memory loss, confusion, cognitive dysfunction, personality changes and other symptoms.

Somatic mutations in the IDH1 gene encoding cytosolic NADP+-dependent isocitrate dehydrogenase, occur after the formation of low-grade glioma and promote tumor progression to glioblastoma. IDH1 gene variants regularly appear in astrocytomas, oligodendrogliomas and oligoastrocytomas. The pathological variations in the IDH2 gene which encoding mitochondrial NADP+-dependent isocitrate dehydrogenase are also discovered in these tumors, however, with lower frequencies. Mutations in TP53, PTEN, CDKN2A and EGFR often occur in different processes of tumor development. For example, in the development of astrocytoma, the TP53 mutation is often detected at an early stage, while the PTEN mutation and EGFR amplification are detected in higher-grade tumors. PTEN is a tumor suppressor gene that negatively regulates the PI3K pathway and mutations in EGFR often cause defects in growth factor signaling pathways. Mutations and genetic rearrangements of BRAF are associated with pilocytic astrocytomas and pleomorphic xanthoastrocytomas.

To support clinical researches, CD Genomics offers a predesigned glioma gene panel sequencing for genes which are frequently mutated and associated with gliomas. Targeted DNA sequencing technology that enables deep sequencing at high coverage levels is provided. Our glioma panel can be applied to efficiently discover, validate and screen genetic variants among the glioma genes and help researchers decode the complexities of glioma biology quickly. In addition, you can choose the panel content from our predesigned library or discuss your custom glioma requirements, then we can provide you with your own panel.


Gene List of Glioma Gene Panel

ATRX BRAF CDKN2A CIC EGFR H3F3A IDH1 IDH2 MDM2 PTEN
TERT TP53                

Highlights of Glioma Gene Panel Sequencing Service

  • Target-enrichment sequencing by Illumina provides extremely high depth (average depth > 1000x) with lower costs.
  • High coverage uniformity ensures the sequencing data more efficiency.
  • SNVs, indels, and even low frequency variations can be detected.
  • Strict quality control throughout the pipeline workflow to ensure the accuracy and repeatability of the sequencing.
  • Fast turnaround time.

Applications of Glioma Gene Panel Sequencing

  • Cancer related mechanism studies
  • Biomarker discovery
  • Therapeutic target discovery
  • Drug target discovery
  • Discover rare mutations and detect low frequency mutations

Service Specifications

Service Specifications

Sample Requirements

  • Sample types for genome DNA from tissues, whole blood, formalin-fixed paraffin-embedded (FFPE) tissues.
  • Recommended amount: 2μg
  • Minimum amount: 200ng
  • Collection: DNA samples are stored in TE buffer or equivalent. Blood is collected by routine blood collection kits.
Service Specifications

Sequencing

  • Illumina PE150
  • Enrichment Method: Amplicon sequencing
  • More than 80% of bases with a ≥Q30 quality score
  • Recommended sequencing depth: ≥ 500x
Service Specifications

Bioinformatics Analysis

We provide customized bioinformatics analysis including:

  • Read alignment
  • Variant calling/Annotation

Gene Panel Workflow

CD Genomics provides the accurate and cost-effective glioma gene panel sequencing and bioinformatics analysis. Our professional expert team executes quality management, following every procedure to ensure confident and unbiased results. The general workflow for glioma gene panel sequencing is outlined below.

Gene Panel Workflow

For more information about the glioma gene panel or need other amplification requirements, please contact us.

References:

  1. Zacher A, et al. Molecular Diagnostics of Gliomas Using Next Generation Sequencing of a Glioma-Tailored Gene Panel. Brain Pathology, 2017. 27, 146-159.
  2. Testa U, et al. Genetic abnormalities, clonal evolution, and cancer stem cells of brain tumors. Medical Sciences, 2018. 6, 85.
  3. Wen P Y, et al. Malignant gliomas in adults. New England Journal of Medicine, 2008. 359, 492-507.
  4. Yan H, et al. IDH1 and IDH2 mutations in gliomas. New England Journal of Medicine, 2009. 360, 765-773.
  5. Hartmann C, et al. Type and frequency of IDH1 and IDH2 mutations are related to astrocytic and oligodendroglial differentiation and age: a study of 1,010 diffuse gliomas. Acta neuropathologica, 2009. 118, 469-474.
* For Research Use Only. Not for use in diagnostic procedures.

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