CD Genomics is one of the professional sequencing companies with extensive experience in detecting gene mutations. We can provide high-quality ARMS-PCR service to assess sequence variations and accelerate disease research.
Amplification refractory mutation system (ARMS) is the development of the application of PCR technology. ARMS-PCR can detect two alleles simultaneously by using a single PCR reaction system, which does not require restriction endonuclease and can distinguish whether the alleles are pure or not. ARMS technology has become one of the international personalized molecular detection techniques for cancer, as well as a good method for mutation detection in genetic diseases such as sickle cell anemia and thalassemia.
ARMS-PCR technology is used to detect known mutant genes. If one allele has a SNP and another alternative is normal, we can analyze the two alleles by designing specific primers for each allele. Two 5'-end primers are designed, one complementary to normal DNA and one complementary to mutant DNA. For homozygous mutations, these two primers and the 3′-end primer are added to perform two parallel PCRs. Only primers that are complementary to the mutant DNA can be extended and PCR amplified products obtained. If the mismatch is located at the 3 'end of the primer, the PCR cannot be extended.
Figure 1. The principle of ARMS-PCR. (Peng et al, 2018)
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