Cancer Hotspot Panel Sequencing

Description

Cancer is induced by the accumulation of harmful DNA mutations in cells. At present, the mechanisms of inducing abnormal cell proliferation, differentiation and cancer production are mainly studied in three categories: activating dominant transforming proto-oncogenes by translocations and mutations, inactivating tumor suppressor gene by mutations, and disordering the DNA repair genes.

CD Genomics provides a predesigned cancer hotspot gene panel sequencing utilizing amplicon sequencing technology by illumina system, which covers a total of 65 common proto-oncogenes and tumor suppressor genes associated with the common human cancer, such as breast cancer, lung cancer and colorectal cancer. And also, you can choose the genes from the gene list to customize your exclusive sequencing and bioinformatics analysis for researches.

Gene List of Cancer Hotspot Panel

ABL1 AKT1 ALK APC ATM BRAF BRCA1 BRCA2 CDH1 CDKN2A
CSF1R CTNNB1 DDR2 DNMT3A EGFR ERBB2 ERBB3 ERBB4 EZH2 FBXW7
FGFR1 FGFR2 FGFR3 FLT3 FOXL2 GNA11 GNAQ GNAS HNF1A HRAS
IDH1 IDH2 JAK2 JAK3 KDR KIT KRAS MAP2K1 MET MLH1
MPL MSH6 MTOR NF1 NF2 NOTCH1 NPM1 NRAS PDGFRA PIK3CA
PIK3R1 PTCH1 PTEN PTPN11 RB1 RET SMAD4 SMARCB1 SMO SRC
STK11 TERT TP53 TSC1 VHL        

Highlights of Cancer Hotspot Panel Sequencing Service

  • Target-enrichment sequencing by Illumina provides extremely high depth (average depth > 1000x) with lower costs.
  • High coverage uniformity ensures the sequencing data more efficiency.
  • SNVs, indels, and structural rearrangement can be detected.
  • Strict quality control throughout the pipeline workflow to ensure the accuracy and repeatability of the sequencing.
  • Fast turnaround time.
  • Provide a list of genes related to cancer hotspots, you can add or delete the genes according to your needs.

Applications of Cancer Hotspot Panel Sequencing

  • Susceptibility screening and cancer risk assessment
  • Therapeutic target discovery
  • Drug target discovery
  • Discover rare mutations and detect low frequency mutations

Service Specifications

Service Specifications

Sample Requirements

  • Sample types for genomic DNA from tissues, whole blood, saliva, formalin-fixed paraffin-embedded (FFPE) tissues.
  • Recommended amount: 2μg
  • Minimum amount: 200ng
  • Collection: DNA samples are stored in TE buffer or equivalent. Blood is collected by routine blood collection kits.
Service Specifications

Sequencing

  • Illumina PE150
  • Enrichment Method: Amplicon sequencing
  • More than 80% of bases with a ≥ Q30 quality score
  • Recommended sequencing depth: ≥ 5,000x
Service Specifications

Bioinformatics Analysis

We provide customized bioinformatics analysis including:

  • Read alignment
  • Variant calling/Annotation
  • Gene expression profiling

Gene Panel Workflow

CD Genomics provides the accurate and cost-effective cancer hotspot panel sequencing and bioinformatics analysis. Our professional expert team executes quality management, following every procedure to ensure confident and unbiased results. The general workflow for cancer hotspot panel sequencing is outlined below.

Gene Panel Workflow

For more information about the cancer hotspot panel or need other amplification requirements, please contact us.

Reference:

  1. Claudio B MD, et al. Young investigator challenge: Can the Ion AmpliSeq Cancer Hotspot Panel v2 be used for next‐generation sequencing of thyroid FNA samples? Cancer CytopathologyVolume, 2016. 124, 776-784.
* For Research Use Only. Not for use in diagnostic procedures.

Online Inquiry
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  • 45-1 Ramsey Road, Shirley, NY 11967, USA
  • 1-631-275-3058 (USA)
           44-208-144-6005 (Europe)
  • 1-631-614-7828
  • info@cd-genomics.com
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