Hereditary Cancer Panel Sequencing

Description

Hereditary cancer panel is a predesigned panel for targeted resequencing assay utilizing amplicon sequencing technology by Illumina system, designed to detect inherited mutations and not suitable for the detection of other types of mutations in acquired cancers. The panel covers a total of 37 genes associated with cancers of the breast, ovary, uterus, prostate, gastric and colorectal, etc.

CD Genomics utilizes targeted NGS sequencing technology to provide more efficient and accurate targeting of specific genes or mutations in hereditary cancer-associated genes. Targeted NGS sequencing facilitates sequencing multiple genes and samples in a single and cost-effective assay.

Gene List of Hereditary Cancer Panel

Exon:

APC BARD1 BMPR1A CDKN2A CHEK2 EPCAM FAM175A GREM1 MEN1 MITF
MLH1 MRE11A MSH2 MSH6 MUTYH NBN PALB2 POLD1 POLE RAD51C
RNF139                  

Coding Sequence (CDS):

ATM BAP1 BLM BRCA1 BRCA2 BRIP1 CDH1 CDK4 PMS2 PTEN
RAD50 RAD51D SMAD4 STK11 TP53 XRCC2      

Highlights of Hereditary Cancer Panel Sequencing Service

  • Target-enrichment sequencing by Illumina provides extremely high depth (average depth > 1000x) with lower costs.
  • High coverage uniformity ensures the sequencing data more efficiency.
  • SNVs, indels, and structural rearrangement can be detected.
  • Strict quality control throughout the pipeline workflow to ensure the accuracy and repeatability of the sequencing.
  • Fast turnaround time.

Applications of Hereditary Cancer Panel Sequencing

  • Cancer risk assessment
  • Personalized health management plan
  • Therapeutic target discovery
  • Drug target discovery

Service Specifications

Service Specifications

Sample Requirements

  • Sample types for genomic DNA from whole blood, tissues, saliva, formalin-fixed paraffin-embedded (FFPE) tissues.
  • Recommended amount: 2μg
  • Minimum amount: 200ng
  • Collection: DNA samples are stored in TE buffer or equivalent. Blood is collected by routine blood collection kits.
Service Specifications

Sequencing

  • Illumina PE150
  • Enrichment Method: Amplicon sequencing
  • More than 80% of bases with a ≥ Q30 quality score
  • Recommended sequencing depth: ≥ 500x
Service Specifications

Bioinformatics Analysis

We provide customized bioinformatics analysis including:

  • Read alignment
  • Variant calling/Annotation

Gene Panel Workflow

CD Genomics provides the accurate and cost-effective hereditary cancer panel sequencing and bioinformatics analysis. Our professional expert team executes quality management, following every procedure to ensure confident and unbiased results. The general workflow for hereditary cancer panel sequencing is outlined below.

Gene Panel Workflow

For more information about the hereditary cancer panel or need other amplification requirements, please contact us.

Reference:

  1. Li J, et al. Germline mutations in 40 cancer susceptibility genes among Chinese patients with high hereditary risk breast cancer[J]. International Journal of Cancer, 2019. 144, 281-289.
* For Research Use Only. Not for use in diagnostic procedures.

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  • 45-1 Ramsey Road, Shirley, NY 11967, USA
  • 1-631-275-3058 (USA)
           44-208-144-6005 (Europe)
  • 1-631-614-7828
  • info@cd-genomics.com
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