•         

Custom CLL Panel

Custom CLL Panel

Introduction

Chronic lymphocytic leukemia (CLL) is a chronic B lymphocyte proliferative disorder characterized by the accumulation of monoclonal and mature small lymphocytes in peripheral blood, bone marrow and lymphoid tissues, and the corresponding clinical symptoms. CLL is common in Western countries, accounting for 65% of leukemia patients over 65 years of age. The incidence rate in the Asia-Pacific region is relatively lower. The median age of onset was 65 to 70 years old. The disease is more common in men, and the ratio of male to female cases is about 2 to 1. The cause of the disease is not fully understood, and environmental factors do not predominate in the pathogenesis of chronic lymphocytic leukemia.

Disease-related gene description

Studies have reported that CLL-related gene variants, not only contain the mutations in TP53 and ATM genes, which are commonly found in many other types of cancer, but also include some new somatic mutations, such as mutations in NOTCH1, SF3B1, XPO1, MYD88, POT1, CHD2, BIRC3, FBXW7 and DDX3X. TP53 encodes a P53 protein, a tumor suppressor that regulates cell division. ATM is involved in DNA repair and cell division, and mutations in this gene result in increased susceptibility to a variety of cancers. NOTCH1 is a tumor protooncogene that encodes a Notch signaling pathway receptor protein, which is involved in cell differentiation, proliferation and survival. The activation mutation of NOTCH1 is associated with CLL. The SF3B1 gene encodes the cleavage factor 3b protein complex subunit 1, which is one of the components of the small nuclear ribonucleoprotein complex. In CLL cases, mutations in the NOTCH1 and SF3B1 genes were detected at a high frequency. Moreover, it has been reported that the variation of NOTCH1 and TP53 abnormalities are often mutually exclusive.

CD-Genomics provides a custom CLL panel platform with amplicon sequencing technology by Illumina MiSeq system/Ion PGM system to help verify the biological effects of the genetic mutations associated with CLL and to research how they affect cellular fitness. From our custom CLL panel, you can select genes which you are interested in, and you can add new genes which are not included in the panel.

Custom CLL panel offers but are not limited to:

  • Targeted amplicon sequencing technology with high sequencing depth and sensitivity by Illumina MiSeq system/Ion PGM system is provided.
  • Strict quality control throughout the pipeline workflow ensures the accuracy and repeatability of the sequencing.
  • Every detected genetic variant will be further validated to ensure the validity of results.
  • Custom panel content is designed to keep up with the frontiers from current literature about CLL panel to target all relevant regions.
  • A custom CLL panel library is provided and you can create your own panel.
  • Precision bioinformatics pipelines ensure superior analytical performance.

Choose the genes that suit you from the chronic lymphocytic leukemia gene list

ATM BIRC3 BRAF BTK
CARD11 CHD2 CDKN2A DDX3X
FBXW7 H1-4 H1-5 IKZF3
IRF4 KRAS MAP2K1 MED12
MYD88 NOTCH1 NRAS PIM1
PLCG2 POT1 PTEN SF3B1
TP53 TRAF2 XPO1 ZMYM3

Specimen requirements of our custom CLL panel

  • Specimen: blood, saliva or extracted DNA.
  • Volume: 2-5 mL blood, 2 mL saliva, 3ug DNA.
  • Collection: blood is collected by routine blood collection and saliva is collected by saliva collection kits (kits are available upon request). DNA samples are stored in TE buffer or equivalent.
  • Container: lavender-top (EDTA) tube or yellow-top (ACD) tube.
  • Storage/transport temperature: room temperature.

Gene panel workflow

Gene panel workflow

For more information about the Custom CLL Panel or need other amplification requirements, please contact us.

References:

  1. Rossi D, et al. Mutations of NOTCH1 are an independent predictor of survival in chronic lymphocytic leukemia. Blood, 2012, 119(2): 521-529.
  2. Rozman C, Montserrat E. Chronic lymphocytic leukemia. New England Journal of Medicine, 1995, 333(16): 1052-1057.
  3. Rodríguez-Vicente A E, et al. Next-generation sequencing in chronic lymphocytic leukemia: recent findings and new horizons. Oncotarget, 2017, 8(41): 71234.
* For Research Use Only. Not for use in diagnostic procedures.

Online Inquiry
  • Verification code
Copyright © 2023 CD Genomics. All rights reserved.
0
Inquiry Basket