Cancer is induced by the accumulation of harmful DNA mutations in cells. At present, the mechanisms of inducing abnormal cell proliferation, differentiation and cancer production are mainly studied in three categories: activating dominant transforming proto-oncogenes by translocations and mutations, inactivating tumor suppressor gene by mutations, and disordering the DNA repair genes. Our custom cancer hotspot gene panel can detect genetic variants in 50 common proto-oncogenes and tumor suppressor genes that participate in the occurrence, development and metastasis of cancer.
The mutations in proto-oncogenes and tumor suppressor genes are generally associated with multiple cancer diseases. Ras pathway genes (NRAS, HRAS and KRAS) are often detected to have genetic variations in a variety of cancers such as colorectal cancers, lung cancers, melanomas and ovarian cancers. At the same time, BRAF mutations are often detected in the above diseases along with RAF mutations. They can activate the same pathway, the RAS–RAF–MEK–ERK–MAP kinase pathway, thereby regulating the response of cells to growth signals. In addition to the above genes, ABL, AKT1, APC, etc. genes in the gene list are proved to be the "driver" genes for cancer.
CD-Genomics provides an optimized custom cancer hotspot gene panel utilizing amplicon sequencing technology by Illumina MiSeq system/Ion PGM system. This panel offers 50 genes associated with the common human cancer, such as breast cancer, lung cancer and colorectal cancer, and you can choose the genes from the cancer hotspot panel gene list to customize your exclusive panel.
For more information about the Custom Cancer Hotspot Panel or need other amplification requirements, please contact us.