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The survival motor neuron (SMN) protein was first highlighted as a protein of interest when mutations in its coding gene, SMN1, were linked to the neuromuscular disease spinal muscular atrophy (SMA), a leading genetic cause of infant mortality. The kit is based on real-time PCR technology to detect Human Survival Motor Neuron 1.
Principle of Test : Based on real-time fluorescent quantitative PCR, which combines competitive PCR, saturated fluorescent dyes, melting curve analysis technology and normalization software processing technology, this kit is used to detect the copy number of SMN1 in DNA samples.
Sample Requirements:
1. Sample: whole blood sample.
2. Collection: 2~5 mL of venous blood is extracted from the subject with a disposable sterile syringe, and injected into a glass tube containing EDTA anticoagulant. The glass tube is immediately reversed gently and mixed for 5~10 times, so that the anticoagulant and venous blood are fully mixed.
3. It is recommended to use a commercial kit to extract human genomic DNA. The extracted DNA needs to be measured with a UV spectrophotometer. When 10ng/μL≤DNA concentration requirement≤120ng/μL, OD260/OD280 is within 1.7~2.0. It is recommended that the extracted DNA be tested immediately, otherwise, please store it below -20℃ for no more than 6 months.
Species: | Human Survival Motor Neuron 1 |
Sample Type: | Whole blood sample |
Storage: | All reagents should be stored at -20℃±5℃ in the dark. The validity period of the reagents is 10 months. The kit should not be frozen and thawed more than 6 times. The opened reagents should be placed at room temperature under the condition of no more than 8 hours. This product is transported under the condition of 0~8℃. Simulated transport tests indicate that the stability and validity could not be influenced by transport, but the transportation time should not exceed 7 days. Production date and expiration date are shown on the packaging label. |
Method: | Real-time PCR |
Equipment Required: | • Gentier 96E real time PCR system • Roche Light Cycler 480 Real-time fluorescence quantitative PCR instrument or similar apparatus |
ShippingCondition: | Dry ice |
Please submit a detailed description of your project. We will provide you with a customized project plan to meet your research requests. You can also send emails directly to info@cd-genomics.com for inquiries.
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