CDCap™ Comprehensive Cancer Panel Kit

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Overview

Product Description

The CDCap™ Comprehensive Cancer Panel is a hybridization capture-based targeted sequencing assay designed for comprehensive cancer genomic profiling. It detects 641 cancer-related genes, covering loci associated with targeted therapeutic mechanisms, chemotherapeutic agent toxicity/sensitivity, immune pathways, and hereditary cancer predisposition. This panel supports precision oncology research, combination therapeutic strategy investigation, and research cohort selection by integrating biomarkers such as TMB, MSI, MMR, alongside PD-1 pathway regulators and hyperprogression-associated genes for immunotherapy response mechanisms analysis.

Features

Key Features & Advantages

Comprehensive Guidance for Precision Therapy
· Evaluates genes linked to targeted/chemotherapy efficacy, immune pathways, and hereditary predisposition.
Multidimensional Prediction of PD-1 Inhibitor Response
· evaluates MSI, TMB, and MMR to comprehensively predict immunotherapy efficacy.
Optimized for Liquid Biopsy
· Streamlined panel design with UMI tagging enhances sensitivity and accuracy for low-input cfDNA detection.

Specifications

Product Specifications

Enrichment Method:	Hybridization Capture
Species:	Human
Variant Types:	SNV、Indel、CNV、Fusion、MSI、TMB
Target Size:	2.1Mb
Cancer Type:	Pan-Cancer
Number of Genes:	641
Sample Type:	Tissue、FFPE、blood
Input DNA/RNA:	cfDNA ≥20ng, gDNA ≥30ng
Method:	NGS
Sequencing Platform:	Illumina
Storage:	Store at -20 °C.

Data

1. Exceptional Correlation: WES and TMB Panel Results Align with High Precision（R² ≥ 0.9966）

2. High Sensitivity and Accuracy in Mutation Detection

Table 1. Mutation Detection Results for Positive Standards

Gene	Variant	Expected Allelic Frequency	Reported allelic frequency - 50 ng	Reported allelic frequency - 100 ng
----	----	----	----	----
EGFR	L858R	1%	0.95%	1.50%
KRAS	A146T	1%	1.02%	0.87%
NRAS	Q61K	1%	0.84%	1.23%
EGFR	T790M	2%	1.61%	2.11%
EGFR	ΔE746_A750	1.58%	1.40%	1.36%
FLT3	ΔI836	2%	2.81%	1.94%
KIT	D816V	2%	2.80%	2.07%
KRAS	G12D	2%	1.32%	2.33%
EGFR	V769_D770 insASV	3%	1.80%	1.02%
EGFR	G719S	4%	3.46%	3.33%
KRAS	G13D	4%	2.94%	4.87%
EML4-ALK	Fusion V3	5%	3.50%	3.61%
CD74-ROS1	Fusion	6%	2.37%	1.61%
BRAF	V600E	7%	5.02%	5.75%
PIK3CA	H1047R	7%	7.28%	5.83%
ERBB2	Amplification	6 copies	6 copies	6 copies

Table 2. SNV Detection Results for 5% FFPE Standards

Gene	Variant	Expected Allelic Frequency	Reported allelic frequency - 50 ng	Reported allelic frequency - 100 ng	Reported allelic frequency - 200 ng
----	----	----	----	----	----
EGFR	L858R	5%	2.48%	6.51%	4.29%
EGFR	T790M	5%	2.64%	3.64%	4.30%
EGFR	ΔE746_A750	5%	2.11%	3.17%	3.37%
KRAS	A146T	5%	2.60%	3.78%	3.88%
KRAS	G12D	5%	1.78%	3.19%	3.77%
KRAS	G13D	5%	1.51%	5.57%	5.93%
NRAS	Q61K	5%	3.36%	5.06%	5.11%
PIK3CA	E545K	5%	1.39%	4.12%	5.73%

All mutations were successfully detected in tumor gDNA and FFPE standards, with reported allelic frequencies closely matching theoretical values.

3. UMI Tagging Enhances Mutation Detection Rate

Table 3. Mutation Detection Results for cfDNA Reference Standards

Gene	Variant	Expected Allelic Frequency	Reported allelic frequency
----	----	----	----
EGFR	L858R	0.50%	0.19%
EGFR	T790M	0.50%	0.35%
EGFR	ΔE746_A750	0.50%	0.27%
KRAS	A146T	0.50%	0.23%
KRAS	G12D	0.50%	0.64%
KRAS	G13D	0.50%	0.76%
NRAS	Q61K	0.50%	0.44%
PIK3CA	E545K	0.50%	0.44%

The use of UMI tagging in cfDNA liquid biopsy significantly reduces background noise, minimizes false-positive mutations, and improves detection accuracy. Libraries were prepared using 15 ng of Horizon 0.5% cfDNA reference standard (HD780) and enriched via liquid-phase hybrid capture with pan-cancer probes.

Gene List

Table1: Gene List

ABCB1	ABCC3	ABL1	ABL2	ACVR1	ACVR1B	AGO2	AKT1	AKT2	AKT3	ALK	ALOX12B
AMER1	ANKRD11	APC	APEX1	AR	ARAF	ARFRP1	ARID1A	ARID1B	ARID2	ARID5B	ASNS
ASXL1	ASXL2	ATIC	ATM	ATR	ATRX	AURKA	AURKB	AXIN1	AXIN2	AXL	B2M
BABAM1	BAP1	BARD1	BBC3	BCL10	BCL2	BCL2L1	BCL2L11	BCL2L2	BCL6	BCOR	BCORL1
BCR	BIRC3	BIRC7	BLM	BMPR1A	BRAF	BRCA1	BRCA2	BRD4	BRIP1	BTG1	BTG2
BTK	C11orf30	C8orf34	CALR	CARD11	CARM1	CASP7	CASP8	CBFB	CBL	CBR3	CCND1
CCND2	CCND3	CCNE1	CD22	CD274	CD276	CD3EAP	CD44	CD70	CD79A	CD79B	CDA
CDC42	CDC73	CDH1	CDK12	CDK4	CDK6	CDK8	CDKN1A	CDKN1B	CDKN2A	CDKN2B	CDKN2C
CEBPA	CENPA	CHD2	CHD4	CHEK1	CHEK2	CIC	CREBBP	CRKL	CRLF2	CSDE1	CSF1R
CSF3R	CTCF	CTLA4	CTNNA1	CTNNB1	CTTN	CUL3	CUL4A	CXCR4	CYLD	CYP17A1	CYP19A1
CYP1B1	CYP2C8	CYP2D6	CYP4B1	CYSLTR2	DAXX	DCUN1D1	DDR1	DDR2	DDX43	DICER1	DIS3
DNAJB1	DNMT1	DNMT3A	DNMT3B	DOT1L	DPYD	DROSHA	DUSP4	DYNC2H1	E2F3	EED	EGFL7
EGFR	EIF1AX	EIF4A2	EIF4E	ELF3	EP300	EPAS1	EPCAM	EPHA2	EPHA3	EPHA5	EPHA7
EPHB1	EPHB4	ERBB2	ERBB3	ERBB4	ERCC1	ERCC2	ERCC3	ERCC4	ERCC5	ERF	ERG
ERRFI1	ESR1	ESR2	ETV1	ETV6	EWSR1	EXT1	EZH1	EZH2	FADD	FAM175A	FAM46C
FAM58A	FANCA	FANCC	FANCD2	FANCE	FANCF	FANCG	FANCL	FANCM	FAS	FAT1	FAT3
FBXW7	FCGR2A	FCGR3A	FGF10	FGF12	FGF14	FGF19	FGF23	FGF3	FGF4	FGF6	FGFR1
FGFR2	FGFR3	FGFR4	FH	FLCN	FLT1	FLT3	FLT4	FOXA1	FOXL2	FOXO1	FOXP1
FRS2	FSHR	FUBP1	FYN	GAB2	GABRA6	GALNT12	GATA1	GATA2	GATA3	GATA4	GATA6
GGH	GID4	GLI1	GNA11	GNA13	GNAQ	GNAS	GPR124	GPS2	GREM1	GRIN2A	GRM3
GSK3B	GSTA1	GSTM1	GSTP1	H3F3A	H3F3B	H3F3C	HAS3	HDAC1	HDAC6	HGF	HIST1H1C
HIST1H2BD	HIST1H3A	HIST1H3B	HIST1H3C	HIST1H3D	HIST1H3E	HIST1H3F	HIST1H3G	HIST1H3H	HIST1H3I	HIST1H3J	HIST2H3C
HIST2H3D	HIST3H3	HLA - A	HLA - B	HMMR	HNF1A	HOXB13	HRAS	HSD3B1	HSP90AA1	HSPB1	ICOSLG
ID3	IDH1	IDH2	IFNGR1	IGF1	IGF1R	IGF2	IKBKE	IKZF1	IL10	IL1A	IL4
IL7R	IL8	INHA	INHBA	INPP4A	INPP4B	INPPL1	INSR	IRF2	IRF4	IRS1	IRS2
JAK1	JAK2	JAK3	JUN	KAT6A	KDM3B	KDM5A	KDM5C	KDM6A	KDR	KEAP1	KEL
KIT	KLF4	KLHL6	KMT2A	KMT2B	KMT2C	KMT2D	KNSTRN	KRAS	LATS1	LATS2	LIG4
LIMK1	LIN28B	LMO1	LRP1B	LTK	LYN	LZTR1	MAF	MAGI2	MALT1	MAP2K1	MAP2K2
MAP2K4	MAP3K1	MAP3K13	MAP3K14	MAPK1	MAPK3	MAPKAP1	MAX	MCL1	MDC1	MDM2	MDM4
MECOM	MED12	MEF2B	MEN1	MERTK	MET	MGA	MGMT	MITF	MKNK1	MLH1	MLH3
MPL	MRE11A	MSH2	MSH3	MSH6	MSI1	MSI2	MST1	MST1R	MTAP	MTHFR	MTOR
MTRR	MUTYH	MXI1	MYC	MYCL	MYCN	MYD88	MYO3B	MYOD1	NBN	NCOA3	NCOR1
NDRG1	NEGR1	NEIL1	NF1	NF2	NFE2L2	NFKBIA	NKX2 - 1	NKX3 - 1	NOS2	NOTCH1	NOTCH2
NOTCH3	NOTCH4	NPM1	NQO1	NQO2	NRAS	NSD1	NT5C2	NTHL1	NTRK1	NTRK2	NTRK3
NUF2	NUP93	OPRM1	P2RY8	PAK1	PAK3	PAK7	PALB2	PARK2	PARP1	PARP2	PARP3
PAX5	PBRM1	PDCD1	PDCD1LG2	PDGFRA	PDGFRB	PDK1	PDPK1	PGR	PHB	PHOX2B	PIK3C2B
PIK3C2G	PIK3C3	PIK3CA	PIK3CB	PIK3CD	PIK3CG	PIK3R1	PIK3R2	PIK3R3	PIM1	PLAT	PLCG2
PLK2	PMAIP1	PMS1	PMS2	PNRC1	POLDI	POLE	PON1	PPARG	PPM1D	PPP2R1A	PPP2R2A
PPP4R2	PPP6C	PRDM1	PRDM14	PREX2	PRKAA1	PRKAR1A	PRKCI	PRKD1	PRKDC	PRSS8	PTCH1
PTEN	PTP4A1	PTPN11	PTPRD	PTPRO	PTPRS	PTPRT	QKI	RAB35	RAC1	RAC2	RAD21
RAD50	RAD51	RAD51B	RAD51C	RAD51D	RAD52	RAD54L	RAF1	RANBP2	RARA	RASA1	RB1
RBM10	RECQL	RECQL4	REL	RET	RFWD2	RHBDF2	RHEB	RHOA	RICTOR	RIT1	RNF43
ROS1	RPS6KA4	RPS6KB2	RPTOR	RRAGC	RRAS	RRAS2	RRM1	RSF1	RTEL1	RUNX1	RUNX1T1
RXRA	RYBP	SDHA	SDHAF2	SDHB	SDHC	SDDH	SEMA3C	SENSN1	SENSN2	SESN3	SETD2
SETD8	SYF3B1	SGK1	SH2B3	SH2D1A	SHOC2	SHQ1	SLCO1B1	SLCO1B3	SLIT1	SLIT2	SLX4
SMAD2	SMAD3	SMAD4	SMARCA4	SMARCB1	SMARCD1	SMO	SMYD3	SNCAIP	SOCS1	SOD2	SOS1
SOX10	SOX17	SOX2	SOX4	SOX9	SPEN	SPOP	SPRED1	SPTAl	SRC	SRSF2	STAG2
STAT3	STAT4	STAT5A	STAT5B	STK11	STK19	STK40	SUFU	SUZ12	SYK	TAF1	TAP1
TAP2	TBX3	TCEB1	TCF3	TCF7L2	TDG	TEK	TERC	TERT	TET1	TET2	TGFB1
TGFBR1	TGFBR2	TIPARP	TLR2	TMEM127	TMPRSS2	TNF	TNFAIP3	TNFRSF14	TNFSF11	TOP1	TOP2A
TP53	TP53BP1	TP63	TRAF2	TRAF7	TRRAP	TSC1	TSC2	TSHR	TSHZ2	TSHZ3	TTF1
TXN	TXNRD2	TYMS	TYRO3	U2AF1	UGT1A1	UGT1A4	UMPS	UPF1	VEGFA	VHL	VTCN1
WHSC1	WHSC1L1	WISP3	WT1	WWTR1	XIAP	XPC	XPO1	XRCC1	XRCC2	XRCC3	YAP1
YES1	ZBTB2	ZFHX3	ZNF217	ZNF703

Table2:Fusion Gene List

NOTCH2	NTRK1	ALK	MSH2	RAF1	ETV5	FGFR3	SLC34A2	PDGFRA	KIT	CD74	ROS1
MYB	EZR	EGFR	BRAF	FGFR1	MYC	NTRK2	RET	FGFR2	KMT2A	ETV6	BRCA2
NUTM1	RARA	BRCA1	ETV4	SDC4	TMPRSS2	BCR	EWSR1	BCL2	RSPO2	MET	ETV1
PPARγ	TERT