CD Genomics is a company with rich experience in panel design and in-silico optimization. There are two targeted sequencing methods available: amplicon-based approaches and hybridization capture-based approaches. The custom panels can provide a rapid, low-cost, ultra-sensitive, high throughput detection for single nucleotide variations (SNVs), insertions/deletions (indels), copy number variations (CNVs) or DNA methylation.
|Hybridization capture-based target enrichment||Amplicon sequencing|
|DNA quantity required||1-250 ng for library prep, 500 ng of library into capture||1-100 ng|
|DNA quality required||Medium-high||Low-medium|
|Number of steps||More steps||Fewer steps|
|Total time||More time||Less time|
|Number of targets per panel||Virtually unlimited by panel size||> 20,000 amplicons|
|Difficulty for panel design||+||+++|
|Target rate and uniformity||++||+++|
|Cost per sample||++1||+|
|Panel development cost||++||+++|
1 A large sample size makes the price more economical.
2 Due to the primer interactions lead to poor extensibility. Each time a primer pair is added to an amplicon sequencing panel, the panel needs to be retested to ensure that all primer pairs still amplify as expected.
CD Genomics provides the accurate and cost-effective custom panel design and optimize. Once you approve the design, your custom panel will be ready for shipment in about 4-6 weeks. The general workflow for custom NGS panel design is outlined below.
For more information about Custom Panel or need other requirements, please contact us.