Custom Panel Product


Brief Introduction

CD Genomics is a company with rich experience in panel design and in-silico optimization. There are two targeted sequencing methods available: amplicon-based approaches and hybridization capture-based approaches. The custom panels can provide a rapid, low-cost, ultra-sensitive, high throughput detection for single nucleotide variations (SNVs), insertions/deletions (indels), copy number variations (CNVs) or DNA methylation.

  • Amplicon sequencing: Specific primers will be designed for PCR amplification for the target region of interest, multiplex up to 20,000+ amplicons in each reaction. Then sequencing adapters are ligated or added by a second PCR amplification and the targeted DNA library is constructed. High-throughput sequencing is then performed.
  • Hybridization capture-based target enrichment: Based on the principle of base complementary pairing, nucleic acid probes will be designed for specific regions of the genome of interest. The target region hybridization enrichment of DNA/RNA library is carried out based on liquid phase environment, and the specific genetic mutation or somatic mutation in the target region are detected by high-throughput sequencing technology.

How to Choose Suitable Approach? You can use the table below to understand the differences between the approaches.

Hybridization capture-based target enrichment Amplicon sequencing
DNA quantity required 1-250 ng for library prep, 500 ng of library into capture 1-100 ng
DNA quality required Medium-high Low-medium
Number of steps More steps Fewer steps
Total time More time Less time
Number of targets per panel Virtually unlimited by panel size > 20,000 amplicons
Difficulty for panel design + +++
Extensibility +++ +2
Target rate and uniformity ++ +++
Cost per sample ++1 +
Panel development cost ++ +++
Throughput Medium High
Best-suited applications
  • Exome sequencing
  • Genotyping
  • Oncology
  • Detecting rare variants
  • Detecting low-frequency somatic variation of single nucleotide polymorphisms (SNPs) and insertions/deletions (indels)
  • Targeted Bisulfite Sequencing
  • Genotyping
  • Oncology
  • Detecting rare variants
  • Detecting disease-associated variants
  • Detecting germline and somatic single nucleotide polymorphisms (SNPs) and insertions/deletions (indels)

1 A large sample size makes the price more economical.
2 Due to the primer interactions lead to poor extensibility. Each time a primer pair is added to an amplicon sequencing panel, the panel needs to be retested to ensure that all primer pairs still amplify as expected.

Workflow

CD Genomics provides the accurate and cost-effective custom panel design and optimize. Once you approve the design, your custom panel will be ready for shipment in about 4-6 weeks. The general workflow for custom NGS panel design is outlined below.

Workflow

Features

  • Fast and streamlined workflow enables library construction in just a few hours.
  • All Kit components are subjected to stringent quality control ensuring the consistency and reproducibility of library preparation.
  • Optimized to deliver high specificity and highly uniform sequencing results.
  • Low-frequency variants can be detected with just a small amount of DNA/RNA input.

For more information about Custom Panel or need other requirements, please contact us.

Reference:

  1. Mariah H, et al. The future is now: Amplicon sequencing and sequence capture usher in the conservation genomics era. Mol Ecol Resour. 2019, 19:795-803.
* For Research Use Only. Not for use in diagnostic procedures.
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