|Product Description:||The substantial sequence diversity of TCRs and BCRs is generated by somatic V(D)J recombination. CDR3 is the preferred target region for many sequencing studies because TCRs of CDR3 can interact directly with peptides and antigens.
CDAMP® Mouse TCR Panel is suitable for Illumina next-generation targeted sequencing (NGS) of T-cell receptor (TCR) and B-cell receptor (BCR) immune repertoire. This kit uses multiplex PCR and UMI technology, and can target enriched multivariate V(D)J regions of the α, β,γ and δ genes, including CDR3. This method greatly improves the uniformity of amplification. It further reduces amplification bias and allows for accurate and sensitive assessment of TCR clonotypes and repertoire diversity.
|Features:||Ready-to-use and containing all necessary reagents for a rapid set-up. Fast and streamlined workflow enables library preparation in just a few hours. Unbiased amplification by multiplex PCR and unique molecular identifiers (UMIs) approaches. Adaptable to low RNA inputs (down to ng).|
|Sample Input Size :||10ng - 1000ng|
|Application:||Suitable for NGS analysis of complex immune repertoire analysis, and the discovery of rare mutations. There are many applications in different fields, including vaccine development, biomarker development immunological disease research, and tumor disease research.|
|Storage:||Stored at –30°C to –15°C|
|Equipment Required:||Illumina MiniSeq / MiSeq / NextSeq 500 / HiSeq / NovaSeq 6000|