|Product Description:||CD NGS Targeted Hotspot Panel is compatible with fresh frozen, and formalin fixed paraffin embedded (FFPE) samples. This assay features high sensitivity and throughput detecting clinically relevant hotspot variants down to 5% with minimal DNA input. With its comprehensive, streamlined workflow, THSP is designed to be seamlessly integrated into any lab. Laboratories with specific challenges, such as low disease specific sample volumes, are able to batch various sample types together and accelerate tumor profiling.|
|Storage:||Store in <-20°C freezer. Repeated thawing and freezing should be avoided.|
|Equipment Required:||Illumina MiniSeq (validated instrument). Illumina MiSeq or NextSeq 500 are also compatible.
Real-time PCR machine (for sample and library QC assays).
Magnetic rack (for amplicon and library clean-up).
|Required Reagents:||Target enrichment.
Library preparation (indexes and adaptor-oligos for Illumina sequencing).
Amplicon and library clean-up (magnetic beads are included).