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CD Respiratory Pathogens Real-time PCR Kit

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RT005 100 tests
OverviewFeaturesSpecificationsTargetReviewsFAQs
Overview

Product Description

In respiratory pathogen studies, timing and accuracy are everything—especially when overlapping symptoms or co-presence of multiple microbes can obscure the picture. The CD Respiratory Pathogens Real-Time PCR Kit offers a streamlined, all-in-one workflow designed for researchers needing dependable, multi-pathogen analysis in a single assay.

Built on advanced real-time fluorescence PCR technology, this kit identifies nucleic acids from 19 respiratory viruses and 6 bacterial species in one reaction. From respiratory syncytial virus and parainfluenza to Klebsiella pneumoniae, the system delivers clear, quantifiable results with minimal user input. It is optimised for use with nasopharyngeal swab samples—making it ideal for high-throughput lab environments.

Why Choose Real-Time PCR for Pathogen Studies?
Real-time fluorescence PCR (qPCR) is a widely used tool in molecular microbiology for its high sensitivity and multi-target capabilities. By using sequence-specific primers and fluorescent probes, researchers can monitor amplification as it happens—removing the guesswork from detection.

Here’s how it works:
• Each probe is labelled with a fluorescent dye (e.g., FAM, HEX) at the 5′ end and a quencher at the 3′ end.
• When the probe hybridises to the target region, the Taq polymerase cleaves the probe, releasing a fluorescence signal.
• Fluorescence intensity rises proportionally with target concentration, allowing for real-time measurement of microbial load.

Features

Key Features & Advantages

Whether you're developing biologics or conducting respiratory pathogen research, this kit offers a high-throughput, low-hassle solution with unmatched performance and usability.
Comprehensive Pathogen Detection: Screens for 25 respiratory targets—19 viral and 6 bacterial species—in a single workflow. This saves time, reduces hands-on steps, and streamlines sample processing.
• High Sensitivity and Accuracy: Engineered to detect extremely low levels of target RNA or DNA, ensuring reliable results even from minimal sample inputs or mixed microbial backgrounds.
• Validated for Specificity: No cross-reaction with 31 commonly encountered organisms, so each positive signal truly reflects the presence of the intended target.
• Integrated Data Visualisation Software: Includes Genetree Viewer for fast, user-friendly result analysis. Supports simplified interpretation and seamless reporting without additional software tools.
• Long-Term Stability: 12-month shelf life ensures consistent reagent performance across extended research timelines or multi-phase projects.

Specifications

Product Specifications

Species: Respiratory viruses
Sequencing Platform: Real-time PCR
Storage: Stable for 12 months at -20℃ (-4°F).
Method: Real-time qualitative PCR
Equipment Required: Real-time PCR thermal cycler (e.g., Bio-Rad CFX96™, ABI QuantStudio®)
ShippingCondition: Dry ice
Technology: Real-time PCR
Shipping Conditions: Dry ice transport
Target

Target Background

Respiratory infections remain a major diagnostic challenge—especially for researchers and CROs navigating monoclonal antibody production tips or pathogen surveillance strategies. The issue lies not just in the variety of pathogens but in how they spread, evolve, and interact.
• Viruses like Influenza A and respiratory syncytial virus (RSV), alongside bacterial threats such as Klebsiella pneumoniae and Streptococcus pneumoniae, are transmitted through droplets, aerosols, and surface contact.
• Their rapid mutation rates and genetic variability complicate detection, especially during seasonal outbreaks or pandemics.
• Co-infections—when patients carry more than one pathogen—further muddy the diagnostic waters.
Traditional tools like microbial culture have served their purpose, but they’re no longer sufficient. These legacy methods are:
• Time-consuming (often taking days to yield results)
• Low in sensitivity
• Inadequate for detecting multiple pathogens simultaneously
Real-Time PCR technology changes the game. By amplifying specific genetic targets, it enables fast, accurate identification of multiple pathogens from a single sample. Whether you're analysing low-copy viral RNA or complex co-infections, PCR offers the reliability and throughput modern diagnostics demand.

Reviews

See What Our Clients Are Saying

FAQs

FAQs

What Pathogens Can This Kit Detect?
How Sensitive Is It?
Is It Compatible with Standard PCR Platforms?
* For research purposes only, not intended for clinical diagnosis, treatment, or individual health assessments.
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