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info@cd-genomics.com
info@cd-genomics.com
The CD KRAS and BRAF RT-PCR Kit is a test product based on real-time PCR technology, which can detect KRAS exons 2, 3, and 4 and BRAF V600E mutations. KRAS and BRAF mutations affect the RAS-RAF-MEK-ERK signaling pathway to regulate cell growth, differentiation, and survival. KRAS and BRAF mutations are typical hallmarks of many types of cancer.
Exon Mutation Nucleotide Change Amino Acid Change COSMIC ID
2 G12D c.35G>A Glycine (G) to Aspartic acid (D) 521
G12C c.34G>T Glycine (G) to Cysteine (C) 516
G12S c.34G>A Glycine (G) to Serine (S) 517
G12R c.34G>C Glycine (G) to Arginine (R) 518
G12A c.35G>C Glycine (G) to Alanine (A) 522
G12V c.35G>T Glycine (G) to Valine (V) 520
G13D c.38G>A Glycine (G) to Aspartic acid (D) 532
3 Q61H c.183A>C & c.183A>T Glutamine (Q) to Histidine (H) 554 & 555
Q61L c.182A>T Glutamine (Q) to Leucine (L) 553
Q61R c.182A>G Glutamine (Q) to Arginine (R) 552
4 K117N c.351A>C & c.351A>T Lysine (K) to Asparagine (N) 19940 & 28519
K117R c.350A>G Lysine (K) to Arginine (R) 1178068
K117E c.349A>G Lysine (K) to Glutamic acid (E) 1360831
A146T c.436G>A Alanine (A) to Threonine (T) 19404
A146P c.436G>C Alanine (A) to Proline (P) 19905
A146V c.437C>T Alanine (A) to Valine (V) 19900
| Sequencing Platform: | Real-time PCR |
| Storage: | Store in < -20℃ freezer. Repeated thawing and freezing should be avoided. |
| Method: | Real-time qualitative PCR |
| Equipment Required: | Real-time PCR instrument (FAM and VIC) |
| Required Reagents: | DNA extraction kit, pipettors and filter tips |
| ShippingCondition: | Dry ice |
Please submit a detailed description of your project. We will provide you with a customized project plan to meet your research requests. You can also send emails directly to info@cd-genomics.com for inquiries.
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