CD Ureaplasma Urealyticum Nucleic Acid Detection Kit

CAT	Size	Inquiry	Basket
RTPK057-01	32 tests
RTPK057-02	96 tests

CAT

Size

Inquiry

Basket

RTPK057-01

32 tests

RTPK057-02

96 tests

Product Infomation
Product Description:	Ureaplasma urealyticum is a species of bacteria that is commonly found in the urogenital tract of both men and women. While it is a normal part of the human microbiome, it can also be responsible for causing infections such as urethritis and prostatitis in men, and pelvic inflammatory disease and preterm birth in women. The kit is based on a PCR reaction to detect ureaplasma urealyticum qualitatively in the sample.
Features:	Principle of Test: The test uses specific primers and probes to detect ureaplasma urealyticum DNA by fluorescence. A synthetic sequence is used as an internal control to monitor the detection process and avoid false negatives. The internal control does not interfere with the target gene or other sequences. Sample Requirements : 1. Sample: male urethral swab samples or female cervical swab samples. 2. Collection container: disposable male swabs or female swabs approved by the state should be used. The swabs should include sleeves, cotton swab rods, and stoppers. The cotton swab rods and stoppers should be firmly connected. 3. Collection: for specific sampling method, please refer to the 'Microbial Specimen Collection Manual'. 3.1 Male: clean the urethral orifice first, and the patient is required not to urinate for 2 hours before sampling. Take urethral secretions or a small cotton swab to extend into the urethra 2~4cm, slightly twist the swab to remove the secretion (it should be slightly mucous), place the secretion or cotton swab into a sterile glass tube and seal it for inspection. 3.2 Female: first use a cotton/linen swab to wipe off the excessive secretions of the cervix, use a vaginal dilator to expand the vagina, then exchange for a cotton swab to extend into the cervix and pass through the epithelial junction until the swab head cannot be seen, rotate for 10-20 seconds, take out the swab, put the swab in a sterile glass tube and seal it for inspection. 4. Storage: samples can be stored at 2~8℃ for no more than 24 hours; under -20℃ for no more than 3 months; under -70℃ for long-time, but repeated freeze-thaw should be avoided. 5. Transportation: use foam box and ice bag to seal for transportation.
Species:	Ureaplasma Urealyticum
Sample Type:	Male urethral swab samples or female cervical swab samples
Storage:	All reagents should be stored at -25ºC~-15ºC. The validity period of the reagents is 12 months. The unused reagents can be sequentially cryopreservation without affecting their stability, but it should not exceed three times for repeated thawing and freezing. The opened reagents should be placed at room temperature under the condition of no more than 8 hours. The products should be transport by ice box or refrigerated truck. Simulated transport tests indicate that the stability and validity could not be influenced by transport, but the transportation time should not exceed 7 days. Production date and expiration date are shown on the packaging label.
Method:	PCR
Equipment Required:	• ABI7500 real time PCR systems • TL988-IV real time PCR system • Gentier 96E real time PCR system • Gentier 96R real time PCR system and Gentier 48E real time PCR system.
ShippingCondition:	Dry ice

Product Infomation

Product Description:

Ureaplasma urealyticum is a species of bacteria that is commonly found in the urogenital tract of both men and women. While it is a normal part of the human microbiome, it can also be responsible for causing infections such as urethritis and prostatitis in men, and pelvic inflammatory disease and preterm birth in women. The kit is based on a PCR reaction to detect ureaplasma urealyticum qualitatively in the sample.

Features:

Principle of Test: The test uses specific primers and probes to detect ureaplasma urealyticum DNA by fluorescence. A synthetic sequence is used as an internal control to monitor the detection process and avoid false negatives. The internal control does not interfere with the target gene or other sequences.
Sample Requirements :
1. Sample: male urethral swab samples or female cervical swab samples.
2. Collection container: disposable male swabs or female swabs approved by the state should be used. The swabs should include sleeves, cotton swab rods, and stoppers. The cotton swab rods and stoppers should be firmly connected.
3. Collection: for specific sampling method, please refer to the 'Microbial Specimen Collection Manual'.
3.1 Male: clean the urethral orifice first, and the patient is required not to urinate for 2 hours before sampling. Take urethral secretions or a small cotton swab to extend into the urethra 2~4cm, slightly twist the swab to remove the secretion (it should be slightly mucous), place the secretion or cotton swab into a sterile glass tube and seal it for inspection.
3.2 Female: first use a cotton/linen swab to wipe off the excessive secretions of the cervix, use a vaginal dilator to expand the vagina, then exchange for a cotton swab to extend into the cervix and pass through the epithelial junction until the swab head cannot be seen, rotate for 10-20 seconds, take out the swab, put the swab in a sterile glass tube and seal it for inspection.
4. Storage: samples can be stored at 2~8℃ for no more than 24 hours; under -20℃ for no more than 3 months; under -70℃ for long-time, but repeated freeze-thaw should be avoided.
5. Transportation: use foam box and ice bag to seal for transportation.

Species:

Ureaplasma Urealyticum

Sample Type:

Male urethral swab samples or female cervical swab samples

Storage:

All reagents should be stored at -25ºC~-15ºC. The validity period of the reagents is 12 months.
The unused reagents can be sequentially cryopreservation without affecting their stability, but it should not exceed three times for repeated thawing and freezing. The opened reagents should be placed at room temperature under the condition of no more than 8 hours. The products should be transport by ice box or refrigerated truck. Simulated transport tests indicate that the stability and validity could not be influenced by transport, but the transportation time should not exceed 7 days. Production date and expiration date are shown on the packaging label.

Method:

PCR

Equipment Required:

• ABI7500 real time PCR systems
• TL988-IV real time PCR system
• Gentier 96E real time PCR system
• Gentier 96R real time PCR system and Gentier 48E real time PCR system.

ShippingCondition:

Dry ice

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