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The CD Colorectal Cancer Gene RT-PCR Panel uses allele-specific primers to detect mutations in KRAS, NRAS, BRAF, PIK3CA, and AKT1 in multiplex PCR reactions.
Gene Exon Amino Acid Change Nucleotide Change Cosmic ID Reaction No.
KRAS 2 G12A c.35G>C 522 1
G12D c.35G>A 521 1
G12R c.34G>C 518 1
G12C c.34G>T 516 1
G12S c.34G>A 517 1
G12V c.35G>T 520 1
G13D c.38G>A 532 1
3 A59T c.175G>A 546 6
A59E c.176C>A 547 6
A59G c.176C>G 28518 6
Q61H c.183A>C 554 4
Q61H c.183A>T 555 4
Q61L c.182A>T 553 4
Q61R c.182A>G 552 4
4 K117N c.351A>C 19940 1
K117N c.351A>T 28519 1
K117R c.350A>G 4696722 1
K117E c.349A>G – 1
A146T c.436G>A 19404 5
A146P c.436G>C 19905 5
A146V c.437C>T 19900 5
NRAS 2 G12D c.35G>A 564 2
G12S c.34G>A 563 2
G12C c.34G>T 562 2
G13R c.37G>C 569 2
G13V c.38G>T 574 2
3 A59T c.175G>A 578 6
A59D c.176C>A 253327 6
Q61K c.181C>A 580 3
Q61L c.182A>T 583 3
Q61R c.182A>G 584 3
Q61H c.183A>C 586 3
Q61H c.183A>T 585 3
4 K117R c.350A>G – 2
A146T c.436G>A 27174 5
BRAF 15 V600E c.1799T>A 476 3
V600E2 c.1799_1800TG>AA – 3
V600D c.1799_1800TG>AT 477 3
V600K c.1798_1799GT>AA 473 3
PIK3CA 9 E542K c.1624G>A 760 4
E545K c.1633G>A 763 4
E545Q c.1633G>C 27133 4
20 H1047R c.3140A>G 775 4
H1047L c.3140A>T 776 4
AKT1 4 E17K c.49G>A 33765 6
Sequencing Platform: | Real-time PCR |
Storage: | Store in < -20℃ freezer. Repeated thawing and freezing should be avoided. |
Method: | Real-time qualitative PCR |
Equipment Required: | Real-time PCR instrument (FAM, ROX, CY5 and VIC) |
Required Reagents: | DNA extraction kit, pipettors and filter tips |
ShippingCondition: | Dry ice |
Please submit a detailed description of your project. We will provide you with a customized project plan to meet your research requests. You can also send emails directly to info@cd-genomics.com for inquiries.
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