CD BRAF Codon 600 RT-PCR Kit

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PN231	64 reactions

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PN231

64 reactions

Product Infomation
Product Description:	The CD BRAF Codon 600 RT-PCR Kit is an assay product that uses specific probes to identify BRAF V600E, V600K, V600D, V600R, V600M, and V600G mutations. The RAF gene encodes a serine/threonine protein kinase that plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell growth and spreading. Mutations in BRAF have been found in cancers including non-Hodgkin's lymphoma, colorectal cancer, malignant melanoma, papillary thyroid carcinoma, non-small cell lung cancer, and lung adenocarcinoma. And BRAF mutations are generally found in tumors with wild-type KRAS, NRAS, and EGFR.
Features:	Detects as little as 1% mutation load. Highly sensitive and specific. Works with FFPE, fresh frozen, FNA and plasma samples. Includes internal control. Available as RUO and CE-IVD. Mutation Name Nucleotide Change Amino Acid Change Frequency V600E c.1799T>A Valine (V) to glutamic acid (E) 70-90% V600K c.1798_1799GT>AA Valine (V) to lysine acid (K) 10-15% V600D c.1799_1800TG>AT Valine (V) to aspartic acid (D) <5% V600R c.1798_1799GT>AG Valine (V) to arginine (R) <5% V600M c.1798G>A Valine (V) to methionine (M) <1% V600G c.1799T>G Valine (V) to Glycine(G) <1%
Sequencing Platform:	Real-time PCR
Storage:	Store in < -20℃ freezer. Repeated thawing and freezing should be avoided.
Method:	Real-time qualitative PCR
Equipment Required:	Real-time PCR instrument (FAM and VIC)
Required Reagents:	DNA extraction kit, pipettors and filter tips
ShippingCondition:	Dry ice

Product Infomation

Product Description:

The CD BRAF Codon 600 RT-PCR Kit is an assay product that uses specific probes to identify BRAF V600E, V600K, V600D, V600R, V600M, and V600G mutations. The RAF gene encodes a serine/threonine protein kinase that plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell growth and spreading. Mutations in BRAF have been found in cancers including non-Hodgkin's lymphoma, colorectal cancer, malignant melanoma, papillary thyroid carcinoma, non-small cell lung cancer, and lung adenocarcinoma. And BRAF mutations are generally found in tumors with wild-type KRAS, NRAS, and EGFR.

Features:

Detects as little as 1% mutation load.
Highly sensitive and specific.
Works with FFPE, fresh frozen, FNA and plasma samples.
Includes internal control.
Available as RUO and CE-IVD.
Mutation Name Nucleotide Change Amino Acid Change Frequency
V600E c.1799T>A Valine (V) to glutamic acid (E) 70-90%
V600K c.1798_1799GT>AA Valine (V) to lysine acid (K) 10-15%
V600D c.1799_1800TG>AT Valine (V) to aspartic acid (D) <5%
V600R c.1798_1799GT>AG Valine (V) to arginine (R) <5%
V600M c.1798G>A Valine (V) to methionine (M) <1%
V600G c.1799T>G Valine (V) to Glycine(G) <1%

Sequencing Platform:

Real-time PCR

Storage:

Store in < -20℃ freezer. Repeated thawing and freezing should be avoided.

Method:

Real-time qualitative PCR

Equipment Required:

Real-time PCR instrument (FAM and VIC)

Required Reagents:

DNA extraction kit, pipettors and filter tips

ShippingCondition:

Dry ice

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