|Product Description:||CD Tumor Structural Variation 5% gDNA Standard is a highly characterized, biologically relevant quality control material covering multiple genetic variants. This standard has been validated by ddPCR for 8 common structural variant loci in the cancer genome, with variant types including copy number variation/amplification, translocation and large fragment insertion/deletion, and insertion/deletion within high/low GC regions.|
(1)Allele Frequency (AF) Genotype is tesed by Droplet Digital PCR, the acceptance criteria as follows：
AF=0%, acceptable range ≤ 0.1%;
AF < 5%, acceptable margin of error = ± 30%;
5% ≤ AF < 10%, acceptable margin of error = ± 20%;
10%≤AF, acceptable error range = ±10%.
(2)Integrity is tesed by Agarose gel electrophoresis, the acceptance criteria as follows：
Bright band of high molecular weight.
SNV: AKT1 E17K,PIK3CA E545K,.
Deletion: EGFR ΔE746_A750.
Insertion: EGFR A767_V769dup.
CNV: MET Amplification, ERBB2 Amplification.
|Method:||Quality Control Method: ddPCR|
|Application:||CD Tumor Structural Variation 5% gDNA Standard is used to evaluate the performance of the NGS process, it helps to revise and monitor your experimental protocols, operational specifications and bioinformatics processes, etc., and to perform a range of subsequent applications.|
|Preservation Solvent:||Tris-EDTA (10mM Tris-HCl, 1mM EDTA), pH 8.0|
|Storage:||Store at 2-8℃, 36 months.|