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CD Tumor Structural Variation 5% gDNA Standard is a highly characterized, biologically relevant quality control material covering multiple genetic variants. This standard has been validated by ddPCR for 8 common structural variant loci in the cancer genome, with variant types including copy number variation/amplification, translocation and large fragment insertion/deletion, and insertion/deletion within high/low GC regions.
Quality Standards
(1)Allele Frequency (AF) Genotype is tesed by Droplet Digital PCR, the acceptance criteria as follows:
AF=0%, acceptable range ≤ 0.1%;
AF < 5%, acceptable margin of error = ± 30%;
5% ≤ AF < 10%, acceptable margin of error = ± 20%;
10%≤AF, acceptable error range = ±10%.
(2)Integrity is tesed by Agarose gel electrophoresis, the acceptance criteria as follows:
Bright band of high molecular weight.
SNV: AKT1 E17K,PIK3CA E545K,.
Deletion: EGFR ΔE746_A750.
Insertion: EGFR A767_V769dup.
Fusion: CD74(6)-ROS1(34),EML4(6)-ALK(20).
CNV: MET Amplification, ERBB2 Amplification.
Method: | Quality Control Method: ddPCR |
Concentration: | 30 ng/μL |
Application: | CD Tumor Structural Variation 5% gDNA Standard is used to evaluate the performance of the NGS process, it helps to revise and monitor your experimental protocols, operational specifications and bioinformatics processes, etc., and to perform a range of subsequent applications. |
Preservation Solvent: | Tris-EDTA (10mM Tris-HCl, 1mM EDTA), pH 8.0 |
Storage: | Store at 2-8℃, 36 months. |
Please submit a detailed description of your project. We will provide you with a customized project plan to meet your research requests. You can also send emails directly to info@cd-genomics.com for inquiries.
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