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CD Tumor Structural Variation 5% gDNA Standard

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OGQC003 1 µg/tube
OverviewFeaturesSpecifications
Overview

Product Description

CD Tumor Structural Variation 5% gDNA Standard is a highly characterized, biologically relevant quality control material covering multiple genetic variants. This standard has been validated by ddPCR for 8 common structural variant loci in the cancer genome, with variant types including copy number variation/amplification, translocation and large fragment insertion/deletion, and insertion/deletion within high/low GC regions.

Features

Key Features & Advantages

Quality Standards
(1)Allele Frequency (AF) Genotype is tesed by Droplet Digital PCR, the acceptance criteria as follows:
AF=0%, acceptable range ≤ 0.1%;
AF < 5%, acceptable margin of error = ± 30%;
5% ≤ AF < 10%, acceptable margin of error = ± 20%;
10%≤AF, acceptable error range = ±10%.
(2)Integrity is tesed by Agarose gel electrophoresis, the acceptance criteria as follows:
Bright band of high molecular weight.


SNV: AKT1 E17K,PIK3CA E545K,.
Deletion: EGFR ΔE746_A750.
Insertion: EGFR A767_V769dup.
Fusion: CD74(6)-ROS1(34),EML4(6)-ALK(20).
CNV: MET Amplification, ERBB2 Amplification.

Specifications

Product Specifications

Method: Quality Control Method: ddPCR
Concentration: 30 ng/μL
Application: CD Tumor Structural Variation 5% gDNA Standard is used to evaluate the performance of the NGS process, it helps to revise and monitor your experimental protocols, operational specifications and bioinformatics processes, etc., and to perform a range of subsequent applications.
Preservation Solvent: Tris-EDTA (10mM Tris-HCl, 1mM EDTA), pH 8.0
Storage: Store at 2-8℃, 36 months.
* For research purposes only, not intended for clinical diagnosis, treatment, or individual health assessments.
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  • 1-631-338-8059
  • 1-631-614-7828
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