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CD Tumor SNV 5% gDNA Standard II

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OGQC002	1 µg/tube

OverviewFeaturesSpecifications

Overview

Product Description

CD Tumor SNV 5% gDNA Standard II is a highly characterized, biologically relevant quality control material covering multiple genetic variants, including 10 SNVs, 2 insertion mutations and 1 deletion mutation and 1 fusion-associated variant validated by ddPCR.

Features

Key Features & Advantages

Quality Standards
(1)Allele Frequency (AF) Genotype is tesed by Droplet Digital PCR, the acceptance criteria as follows：
AF=0%, acceptable range ≤ 0.1%;
AF < 5%, acceptable margin of error = ± 30%;
5% ≤ AF < 10%, acceptable margin of error = ± 20%;
10%≤AF, acceptable error range = ±10%.
(2)Integrity is tesed by Agarose gel electrophoresis, the acceptance criteria as follows：
Bright band of high molecular weight.

SNV: BRAF V600E,EGFR L858R,EGFR T790M,EGFR G719A,KRAS G12C,KRAS G13D,NRAS Q61R,PIK3CA H1047R,PIK3CA E545K, FGFR3 Y375C.
Deletion: EGFR ΔE746_A750del, MET Exon 14 Skipping.
Insertion: EGFR A767_V769dup,ERBB2 A775_G776insYVMA.

Specifications

Product Specifications

Method:	Quality Control Method: ddPCR
Concentration:	30 ng/μL
Application:	CD Tumor SNV 5% gDNA Standard II is used to evaluate the performance of the NGS process and to help revise and monitor your protocols, protocols and bioinformatics processes for a range of subsequent applications.
Preservation Solvent:	Tris-EDTA (10mM Tris-HCl, 1mM EDTA), pH 8.0
Storage:	Store at 2-8℃, 36 months.

* For research purposes only, not intended for clinical diagnosis, treatment, or individual health assessments.

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