|Product Description:||CD Tumor SNV 5% gDNA Standard II is a highly characterized, biologically relevant quality control material covering multiple genetic variants, including 10 SNVs, 2 insertion mutations and 1 deletion mutation and 1 fusion-associated variant validated by ddPCR.|
(1)Allele Frequency (AF) Genotype is tesed by Droplet Digital PCR, the acceptance criteria as follows：
AF=0%, acceptable range ≤ 0.1%;
AF < 5%, acceptable margin of error = ± 30%;
5% ≤ AF < 10%, acceptable margin of error = ± 20%;
10%≤AF, acceptable error range = ±10%.
(2)Integrity is tesed by Agarose gel electrophoresis, the acceptance criteria as follows：
Bright band of high molecular weight.
SNV: BRAF V600E,EGFR L858R,EGFR T790M,EGFR G719A,KRAS G12C,KRAS G13D,NRAS Q61R,PIK3CA H1047R,PIK3CA E545K, FGFR3 Y375C.
Deletion: EGFR ΔE746_A750del, MET Exon 14 Skipping.
Insertion: EGFR A767_V769dup,ERBB2 A775_G776insYVMA.
|Method:||Quality Control Method: ddPCR|
|Application:||CD Tumor SNV 5% gDNA Standard II is used to evaluate the performance of the NGS process and to help revise and monitor your protocols, protocols and bioinformatics processes for a range of subsequent applications.|
|Preservation Solvent:||Tris-EDTA (10mM Tris-HCl, 1mM EDTA), pH 8.0|
|Storage:||Store at 2-8℃, 36 months.|