Current Status of TMB Research

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Tumor Mutation Burden (TMB) Reference Products Details

TMB is determined by sequencing the DNA of tumor cells and comparing it to DNA from healthy cells from the same patient. The number of mutations in the tumor DNA is then divided by the total size of the DNA sample to calculate TMB. We develop accurate TMB reference products for use in experimental procedures or quality control of the corresponding kits.

Origin

Our TMB reference products are derived from cell lines that mimic patient samples.

Production Process

The production process is specified as follows:

(1) Tumor cells and leukocytes from patient samples are obtained and passaged.

(2) TMB controls in the form of FFPE, gDNA, and ctDNA are mixed in certain proportions.

(3) The TMB controls are sequenced by WES and the TMB values of paired samples are calculated.

(4) Validated by ddPCR to ensure accurate and stable mutation frequency for each batch of generated reference.

Product Highlights

• Cell-derived controls that highly mimic patient samples.
• Stable and consistent results tested by a professional NGS testing company.
• Pairs of controls are available to filter for germline mutations.
• Complete WES sequencing data, TMB reference values, and raw signal process.
• Paired controls TMB values cover multiple gradients from 2 to 28.
• Clinical samples with different tumor contents can be simulated by mixing tumor and normal cell ratios.

Applications

• Reference products for corresponding molecular assays.
• For optimization and validation of new TMB assay procedures or kits.
• To test the sensitivity, accuracy and specificity of TMB sequencing methods.
• Compare the differences between platforms, such as WES, targeted sequencing panels and gene expression profiling.