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Single-Virus Genome Sequencing


Viruses are one of the most abundant biological entities on earth and an important reservoir of genetic diversity that are only beginning to be elucidated for certain environments. Single-cell sequencing technologies has enabled sequencing of individual genomes of eukaryotes, prokaryotes, as well as viruses. We provide robust single-virus genome sequencing to reveal the genetic heterogeneity of viruses and reconstruct accurate & complete viral genomes.

Our Advantages:
  • Standardized laboratory methods and quality assurance systems.
  • Streamlined single-virus sorting, DNA isolation, whole genome amplification, barcoded library generation and sequencing.
  • The most advanced microbial single-cell sequencing technologies and bioinformatics tools.
Tell Us About Your Project

We are dedicated to providing outstanding customer service, listening to customer requests and being reachable at all times.

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Introduction to our single-virus genome sequencing

One of the major bottlenecks in metagenomics is to obtain accurate and complete viral genomes. Normally, widely diverse viral populations are harvested with unequal abundance for metagenomics, achieving coverages below 2-5×. Here we provide single virus genomics that is complementary to this method to accurately reconstruct complete single viral genomes. Single virus genome sequencing can untangle the genetic complexity of microbial communities through sequencing the genome of each viral particle. Single-virus genome sequencing can be used to discover novel viruses, detect emergent pathogenic viruses, reveal natural mutations, assign taxonomic identity and understand viral evolution.

Our single-virus genome sequencing includes seven steps, sample preparation, single virus sorting (via flow cytometry, fluorescence-activated virus sorting, confocal microscopy), viral DNA isolation (using commercial kits), whole genome amplification (via WGA, commonly multiple displacement amplification, MDA or Multiple annealing and looping–based amplification cycles, MALBAC), library construction, multiplexing sequencing (via Illumina HiSeq/MiSeq, PE 150/250), and data analysis. Following each procedure, we will perform strict quality control and provide a quality report.

Single-virus genome sequencing workflow

Bioinformatics Analysis

Our bioinformatics analyses generally include read QC, genome assembly, genome QC and functional annotation. Our bioinformatics analysis pipeline is flexible to your needs. Please feel free to contact us.

Bioinformatics Analysis Details
Read QC Read quality assessment, sequence trimming and filtering using tools like FastQC
Genome Assembly Genome assembly using tools like SPAdes
Genome QC Assessing the quality, contamination and continuity of assembled genome using PCR and tools like ProDeGe program and BLAST
Functional Annotation Prediction and annotation of open reading frames (ORFs) and structural viral proteins
Variant Calling Detection of SNPs/InDels/SVs
Custom Analysis More data analysis services customized to your needs

Sample requirement

Sampling kits: we provide a range of microbial sampling kits for clients, including MicroCollect™ oral sample microbial collection products and MicroCollect™ stool sample collection products.

Deliverables: raw sequencing data, assembled and annotated sequences, quality-control dashboard, and the customized bioinformatics report.

References

  1. Martinez-Hernandez F, Fornas O, Gomez M L, et al. Single-virus genomics reveals hidden cosmopolitan and abundant viruses.
  2. Allen L Z, Ishoey T, Novotny M A, et al. Single virus genomics: a new tool for virus discovery. PloS one, 2011, 6(3): e17722.

Areas of Interest

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