Viruses are one of the most abundant biological entities on earth and an important reservoir of genetic diversity that are only beginning to be elucidated for certain environments. Single-cell sequencing technologies has enabled sequencing of individual genomes of eukaryotes, prokaryotes, as well as viruses. We provide robust single-virus genome sequencing to reveal the genetic heterogeneity of viruses and reconstruct accurate & complete viral genomes.
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One of the major bottlenecks in metagenomics is to obtain accurate and complete viral genomes. Normally, widely diverse viral populations are harvested with unequal abundance for metagenomics, achieving coverages below 2-5×. Here we provide single virus genomics that is complementary to this method to accurately reconstruct complete single viral genomes. Single virus genome sequencing can untangle the genetic complexity of microbial communities through sequencing the genome of each viral particle. Single-virus genome sequencing can be used to discover novel viruses, detect emergent pathogenic viruses, reveal natural mutations, assign taxonomic identity and understand viral evolution.
Our single-virus genome sequencing includes seven steps, sample preparation, single virus sorting (via flow cytometry, fluorescence-activated virus sorting, confocal microscopy), viral DNA isolation (using commercial kits), whole genome amplification (via WGA, commonly multiple displacement amplification, MDA or Multiple annealing and looping–based amplification cycles, MALBAC), library construction, multiplexing sequencing (via Illumina HiSeq/MiSeq, PE 150/250), and data analysis. Following each procedure, we will perform strict quality control and provide a quality report.
Our bioinformatics analyses generally include read QC, genome assembly, genome QC and functional annotation. Our bioinformatics analysis pipeline is flexible to your needs. Please feel free to contact us.
|Read QC||Read quality assessment, sequence trimming and filtering using tools like FastQC|
|Genome Assembly||Genome assembly using tools like SPAdes|
|Genome QC||Assessing the quality, contamination and continuity of assembled genome using PCR and tools like ProDeGe program and BLAST|
|Functional Annotation||Prediction and annotation of open reading frames (ORFs) and structural viral proteins|
|Variant Calling||Detection of SNPs/InDels/SVs|
|Custom Analysis||More data analysis services customized to your needs|
Sampling kits: we provide a range of microbial sampling kits for clients, including MicroCollect™ oral sample microbial collection products and MicroCollect™ stool sample collection products.
Deliverables: raw sequencing data, assembled and annotated sequences, quality-control dashboard, and the customized bioinformatics report.