Targeting Induced Local lesions IN Genomes (TILLING) is a novel approach to reverse genetics research, which enables rapid and efficient identification of point mutations from mutagenized populations of chemical mutagens (EMS) by means of high-throughput assays. TILLING is currently used in the study of a wide range of organisms.

CD Genomics provides high quality TILLING services and is effectively used to explore plant gene function. We combine random chemical mutagenesis with efficient screening techniques for identifying mutations to provide a range of mutations that eliminate or reduce gene function and are used to help researchers gain a better understanding of plant biology and non-GM improved crops. To date, we have used TILLING to complete screens in a variety of plants, such as rice, tomato, rape, lotus, wheat, and pea.

Technical features

• Simple technology

Since TILLING technology is an organic combination of traditional chemical mutagenesis and two-color infrared fluorescence detection, it is a high-throughput and relatively inexpensive technology that can be used for large-scale screening of mutant libraries without complex techniques and equipment, improving screening efficiency.

• Predictable mutation frequency and satisfactory mutation density with a small population

TILLING technology can estimate the probability of mutation in advance and guide the construction of mutant libraries.

• High-throughput, automated operation allows rapid identification results

TILLING technology integrates automatic spiking equipment, high-throughput electrophoresis detection equipment, and excellent image processing and analysis system, which easily automates the operation.

• It overcomes the limitations of knockout methods

The method is simple and efficient, using chemical mutagenesis to obtain the traditional point mutation allele series of all genes.

TILLING can be applied in the following studies

TILLING relies on traditional chemical mutagenesis and is applicable to most crops. Through high-throughput sequencing technology, TILLING has important applications in identifying single nucleotide polymorphisms (SNPs) in genes of interest, screening natural populations for spontaneous mutations, and identifying mutations in specific genes.

• Gene Discovery
• DNA polymorphism assessment
• Plant Improvement

TILLING is widely used in the field of crop breeding research and is an important method for functional genomics research and breeding. And it is effectively applied to more and more biological species, and its important value in genetic research is increasingly reflected.

Our service workflow

Our advantages and features

• High-throughput sequencing platform to ensure the accuracy of experimental data.
• One-stop experimental service to maximize the efficiency of experiments.
• Flexible customization of experimental protocols to meet the various requirements of customers in the progress of the project.
• Timely updates on project progress, timely feedback on projects, no worries for customers.

CD Genomics continuously optimizes the service process, improves the quality of service, helps customers to develop the most scientific and reasonable experimental plan, and promises to provide customers with the most fair and reliable data results. If you are interested in us, please feel free to contact us.