T-DNA Insertion Analysis

T-DNA Insertion Analysis


T-DNA Insertion Analysis

T-DNA insertion is a widely used technique for gene knockout and studying gene function in plants. T-DNA is a segment of DNA derived from the Ti plasmid of Agrobacterium tumefaciens, a soil bacterium commonly used in plant genetic engineering and precision breeding. The T-DNA segment contains genes responsible for transferring itself from the bacterium into the host plant's genome. With the advancements in sequencing technology, the use of next-generation sequencing (NGS) and long-read sequencing has revolutionized the analysis of complex T-DNA insertion strains.

CD Genomics provides a faster, more comprehensive, and cost-effective approach to revolutionize your understanding and utilization of genetic modifications in crop species. Our T-DNA insertion analysis service aids in the identification of T-DNA insertion sites and the assessment of any potential structural variations, such as rearrangements or duplications, associated with the insertions.

CD Genomics is fully dedicated to employing state-of-the-art sequencing technology for driving innovation and make significant contributions to agricultural research and crop improvement.

Benefits of Our T-DNA Insertion Analysis Service

  • Characterization of T-DNA integration events: Sequencing provides detailed information about the structure and composition of T-DNA insertion events. It helps determine the orientation, copy number, and potential rearrangements of T-DNA within the host genome.
  • Identification of precise insertion sites: The precise determination of the T-DNA insertion sites within the plant genome. This information is crucial for understanding the genetic changes and potential effects on nearby genes.
  • Genome-wide analysis: A comprehensive analysis of T-DNA insertion events across the entire plant genome. We allow for the identification of multiple insertion sites, revealing patterns of T-DNA integration and potential hotspots.
  • Assessment of genomic context: By analyzing the sequencing data, you can investigate the genomic context surrounding the T-DNA insertion sites. This includes identifying neighboring genes, regulatory elements, and potential effects on gene expression and phenotype.
  • Functional analysis of gene disruption or activation: T-DNA insertion analysis can provide insights into the functional consequences of gene disruption or activation caused by T-DNA integration. By identifying the affected genes and their genomic location, you can investigate their role in biological processes and pathways.
  • Development of mutant resources: T-DNA insertion analysis based on sequencing allows for the identification of T-DNA insertion mutants. These mutants can be valuable resources for functional genomics studies, enabling you to investigate gene function and phenotype.
  • Improvement of transformation techniques: We can help evaluate and optimize T-DNA delivery methods and transformation protocols. Understanding the patterns and preferences of T-DNA integration can aid in the development of more efficient and targeted transformation techniques.

Instructions for Providing Samples

Sample Type Genomic DNA or Plants tissue
Sample Concentration DNA concentration ≥ 20 ng/µL
Total Sample DNA > 500 ng
Green tissue ≥ 5 g
Sample Purity OD260/280: 1.8~2.0
Electrophoresis requirements No degradation or contamination

Workflow of T-DNA Insertion Analysis

Fig2: Workflow of T-DNA Insertion Analysis – CD Genomics

Our Advantages and Features

  • Gene Function Studies: Vital for understanding the underlying molecular mechanisms involved in important agricultural traits, such as disease resistance, abiotic stress tolerance, and yield improvement.
  • Trait Mapping and Marker Development: Identify genomic regions responsible for those traits. We enable the development of molecular markers that can be used to expedite breeding programs and accelerate the selection of desirable traits in crops.
  • Crop Improvement and Precision Breeding: Help you strategically design breeding programs to introduce or modify genes of interest, such as the development of improved crop varieties with enhanced agronomic traits, nutritional quality, or stress tolerance.
  • Pathway Engineering and Metabolic Engineering: Help you modify or enhance the production of valuable compounds, such as secondary metabolites, pharmaceuticals, or biofuels.
  • Crop Resilience and Stress Response: Our service can be applied to develop crop varieties capable of withstanding various biotic and abiotic stresses, contributing to more sustainable and resilient agriculture.
  • Genomic Selection and Predictive Breeding: Genomic selection enables the prediction of complex traits and facilitates the selection of superior genotypes at an early stage. This accelerates the breeding process, resulting in the development of improved crop varieties with desirable traits.
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