Exome sequencing plays an important role as a research tool to study genetic variation and rare variants in the life sciences. Combined with high-throughput sequencing, sequencing analysis of whole-genome exome regions captured and enriched using efficient sequence capture technology can directly identify genetic variants associated with protein functional variation and successfully detect nucleotide changes in target coding regions in plant and animal studies. In addition, exome sequencing can accelerate the localization of genes for important traits such as sex, resistance and growth in plants, which has promising applications.

CD Genomics provides a rapid, one-stop solution for exome sequencing from your samples through data analysis. We offer a complete solution from experimental design to bioinformatics analysis to help you sequence your targets in depth, with high coverage, without the high costs like whole genome sequencing. We offer exome sequencing technology that has now yielded numerous results in plant and animal related studies, such as our exome study of pigs, where we identified genes involved in neonatal embryonic mortality in pigs based on nucleotide changes and litter rates. We sequenced soybean seed exomes and identified genes related to the control of fatty acid desaturases. Our exome sequencing services will provide research strategies and lay the foundation for subsequent research in genetics.

Application of exome sequencing in agriculture

• Assessing natural plant evolution
• Studying host pathogen interactions
• Improving crop production
• Developing important plant and animal genomic resources
• Plant and animal genetic breeding research

Advantages of exome sequencing

• Direct sequencing of protein coding sequences to identify variants that affect protein structure.
• Achieves comprehensive coverage of coding regions with high-depth sequencing, which can identify common variants and rare variants with frequencies below 1%.
• Sequencing for exome regions provides an economical alternative to whole-genome sequencing, effectively reducing cost, cycle time, and workload.
• Identifies variants in a variety of applications.

Instructions for providing samples

• Sample type non-degradable, non-contaminated DNA samples
• Sample requirement ≥ 2 μg
• Sample concentration ≥ 30 ng/μL
• Sample purity OD 260/280 = 1.8-2.0

Exome sequencing workflow

Fig 2. Exome sequencing workflow

Our advantages and features

• Shorter turnaround time and better cost performance. We have fast library preparation and exon enrichment capabilities to save your research time.
• Accurate data results, providing ultra-fast analysis. Our professional sequencing platform and experienced technical team are committed to provide you with the most reliable experimental results.
• Flexible customization. We will provide the best experimental solution to meet your needs according to your project requirements.
• Wide range of services. We take on a wide variety of your sample materials.

CD Genomics offers a whole exome sequencing workflow that streamlines the entire process from exome enrichment and library preparation to sequencing, data analysis and biological interpretation, helping researchers to gain a comprehensive view of gene regulation, if you are interested in us, please feel free to contact us.