ChIP-seq, a genome-wide DNA binding method for identifying transcription factors and other proteins, is widely used to study protein-DNA interactions in animals and plants. According to the ChIP protocol, DNA-binding proteins are immunoprecipitated using specific antibodies. The bound DNA is then co-precipitated, purified and sequenced, allowing efficient and accurate genome-wide screening and identification of DNA binding sites for specific proteins, laying the foundation for further research to reveal the gene regulatory pathways at play in various plant and animal diseases and biological pathways

CD Genomics has extensive experience in ChIP-seq services in animal and plant research, solving the challenges of ChIP-seq application in plants. We provide high-quality antibodies for target transcription factors, as well as construct transgenic plants expressing tagged proteins, overcoming the technical obstacle of long cycle time and high failure rate of preparing antibodies and transgenic materials for ChIP-seq application in plants. This service of ours is equally effective in animal research. Based on ChIP-seq research, we will provide research strategies to improve agricultural productivity, farming and resistance of plants and animals to diseases.

Advantages of ChIP-seq technology

ChIP-Seq does not require known sequence probes and provides genome-wide analysis through massively sequencing, generating millions of counts in multiple samples for cost-effective, accurate, and unbiased investigation of epigenetic patterns. Other advantages include.

• Wide range of species. Experience in enriching multiple tissues such as flowers, leaves, endosperm.
• Minimal library building. Only 5ng or more of DNA after immunoprecipitation is required to start sequencing analysis.
• Flexible protocols. Different histone modification-specific antibodies are selected according to different experimental needs.

Our ChIP-seq services can be applied in the following research

• Study of the evolutionary genomics of chordates and other marine organisms for species studies.
• Genome-wide DNA binding site detection in plants.
• Genome-wide identification of TF (transcription factor) binding sites and target genes.
• Genome-wide protein marker assays.
• Genome-wide analysis of RNA polymerase distribution.
• Improvement of sequencing of repetitive genomic sequences.
• Annotation of genomes with possible deletions.

Instructions for providing samples

ChIP-seq workflow

Fig 2. ChIP-Seq workflow

Our advantages and features

• High quality data results to ensure the most reliable experimental data support.
• Full suite of sequencing services, we perform advanced data analysis of your ChIP-seq data.
• Integrated workflow to simplify sequencing from library preparation to data analysis.
• Customized protocols, we customize sequencing reagents and protocols according to your experimental needs.

CD Genomics provides the best quality ChIP-seq service in the field of plant and animal research. We have a professional sequencing technology team, the most advanced sequencing platform, and promise to provide you with the fastest experimental turnaround rate and the most accurate and reliable experimental results. If you are interested in us, please feel free to contact us.