SNP Detection
Single Nucleotide Polymorphisms (SNPs) are single nucleotide variants in the genome, which are point mutations caused by the conversion, reversal, insertion or deletion of a single base at a specific nucleotide position in the genomic DNA, resulting in differences between populations and between individuals. Of these, conversions and reversals occur more frequently.
CD Genomics has accumulated a wealth of experience in SNP site analysis and detection. Not only do we have advanced instrumentation, but we have also designed and developed a variety of assays, designed and developed a variety of assays, and the following are the SNP detection services we currently offer.
Types of SNP detection
- 2b-RAD services
- ddRAD-seq services
- Genotyping by sequencing (GBS)
- DNA typing services
- Ligase detection reaction (LDR)
- Multiplex SNaPshot
- TIILLING
- Reduced-Representation Library (RRL)
- Restriction Site Associated DNA Sequencing (RAD-Seq)
Technical characteristics
- High number of SNPs, wide distribution and high density
- Genetic stability
- SNPs are suitable for rapid, scaled-up screening
- Easy estimation of SNP allele frequencies
- Easy to genotype
- Easy to automate analysis
SNP detection can be applied to the following research areas
Due to their high number and frequency of occurrence, SNPs have high research value and application prospects in the following research areas.
- Research on genetic variation in plants and animals
- Plant and animal evolution research
- Microbiological research
- Ecological research
- Crop breeding
- Plant and animal genetic association analysis
- Localisation of important genes in plants and animals
Instructions for providing samples
| SNP locus information | The client will need to provide the rs number of the SNP site, and for those without rs numbers, e.g. cattle, chicken, fish, the exact sequence of 200 bp upstream and downstream of the SNP site, the type of mutation at the SNP site, and whether there are other sites within 25 bp upstream and downstream of the site | |
|---|---|---|
| Typing samples | Genomic DNA | DNA concentration ≥20 ng/μL, volume >10 μL, OD 260/OD 280 = 1.7-1.9 |
| Blood sample | For volumes greater than 500 μL, anticoagulant is required. Please take care to keep the sample at a low temperature during delivery to prevent DNA degradation. Blood spot samples, two or more spots of 9-25 mm2 in size | |
| Sending samples | Place an adequate amount of ice packs in the sample package to reduce the possibility of DNA degradation due to temperature changes during transport | |
Service workflow
Fig 2. SNP detection workflow
Our advantages and features
- Personalised service. We have a wealth of experience in SNP genotyping and can design a personalised test according to your needs.
- Fast cycle times. We have a wide range of testing platforms for fast and stable SNP genotyping.
- Cost-effective. We offer the most comprehensive and professional service at the best price.
CD Genomics provides SNP detection services with short service cycle and high accuracy, which have been widely used in genetic diversity analysis, phylogenetic analysis and molecular breeding research so far. If you are interested in us, please feel free to contact us.
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