Polymerase Chain Reaction (PCR) has an important role in agricultural science research and is commonly used for gene cloning, genetic analysis, and diagnosis of plant and animal diseases. Once its specific primers are obtained, the subject can be judged in the shortest possible time.

CD Genomics provides animal and plant custom PCR services for large DNA fragments, difficult-to-amplify complex templates, low-abundance DNA, and more that you face in agrigenome research. Our professional technology platform and experienced molecular biology experts can amplify the target fragments you need at the maximum rate. We can solve your complex and time-consuming PCR optimization problems. Our custom PCR services will save you valuable research time, improve your research efficiency.

Components and reagents needed for PCR equipment

• DNA target region or DNA template
• DNA polymerase
• Primers
• Deoxyribonucleoside triphosphate, or dNTP
• Buffer solution
• bivalent cations, typically magnesium (Mg) or manganese (Mn) ions

Reaction procedure

Conventional PCR amplification procedures include denaturing the DNA template at 94-96 °C, 50-60 °C to promote sufficient annealing of primers to the template, and then 72 °C to extend the primers on the template to synthesize DNA. In addition, in each cycle, the temperatures used and the length of time they are applied depend on various parameters, including the enzymes used for DNA synthesis, the concentrations of divalent ions and dNTPs in the reaction, and the melting temperature (T_m) of the primers.

CD Genomics offers custom PCR reactions that, by optimizing the PCR process, we can provide as follows.

• Depending on the complexity of the amplified template, fragment size, our molecular biology experts design a specific reaction program for you to maximize experimental success by optimizing the PCR reaction procedure.
• According to your template conditions, we synthesize specific primers for you.
• We have specific amplification enzymes for large fragment templates, complex templates, difficult to amplify templates, and templates with high CG content to ensure maximum PCR amplification efficiency.

Fig 2. PCR reaction process.

Service features and advantages

• Thoughtful service. The whole process of personal service, service the country.
• More professional. We have the most professional technical team, focusing on providing customized PCR services for customers.
• High efficiency. Professional technical platform, experienced technical team, the shortest time to deliver your experimental results.
• More accurate. We have the most advanced PCR experimental platform, to provide you with the most accurate experimental results.

CD Genomics offers animal and plant custom PCR services that can solve the problem of difficult to amplify or false positive target fragment DNA during our customers' research experiments. Our experienced technical team will customize a professional PCR amplification plan for your experimental needs, optimize the PCR process, and solve your experimental challenges. If you are interested in us, feel free to contact us.