Double digest RAD sequencing (ddRAD-seq) is the RAD-derived sequencing strategy in which genomic DNA is digested by combining a restriction endonuclease with a common enzyme that recognises fewer sites and another enzyme that recognises more sites, eliminating the need for physical interruption and allowing direct screening of the target fragment and the introduction of an individual recognition tag (index) near the second site by PCR amplification, allowing more samples to be mixed together for high-throughput sequencing, a research method widely used for SNP discovery and genotyping.

CD Genomics offers high quality ddRNA-seq services to help our clients to better and steadily extract the genomes of plant and animal species to be tested. Our efficient, high quality ddRAD sequencing methods can effectively reduce and simplify the complexity of the genomes of the species to be tested, achieve genetic diversity, and allow for large scale screening and identification of species without a reference genome, regardless of the genome sequence. As a result, we have a significant advantage in improving the accuracy and stability of identification results, and our services reduce the cost of experimentation to meet the needs of our clients' cost-effective projects. Our ddRNA-seq service is dedicated to helping clients discover new SNPs, providing data support for the identification of new plant and animal species, and promoting innovation in agriculture.

Advantages of ddRAD-seq technology

• Multiple samples can be reused
• Simpler and faster library preparation workflow
• Consistent fragment length across samples at the same digestion site
• Flexibility in SNP density
• High sequence coverage
• Low experimental costs
• No need for a reference genome

ddRAD-seq can be used in the following studies in plants and animals

• Genetic library construction for plant and animal traits
• Precision breeding of plants and animals
• Identification of plant and animal varieties
• High-density genetic mapping
• Locating highly differentiated regions and functional genes
• Screening for molecular markers
• Genetic analysis of plant and animal populations

Workflow of ddRAD-seq

Fig 2. Workflow of ddRAD-seq

Our advantages and features

Using an advanced multi-technology platform. We provide our customers with reliable data results while meeting their cost-efficiency requirements.

Technical support services. Meeting all the needs of our customers in their project studies

Simplify the experimental process and ensure high turnaround. Multi-locus projects can be converted into multiplexed assays and typing can be completed in one tube.

Real-time presentation of project progress. Customers can clearly understand every step of the project progress.

Flexibility to tailor experimental protocols. Our experts will tailor the most appropriate protocol to your project needs and characteristics.

CD Genomics is committed to providing high quality and efficient ddRAD-seq services based on our solid expertise and advanced instrumentation platform. Our ddRAD-seq method has been used with great success in more than a dozen important crops. If you are interested in us, please feel free to contact us.