CD Genomics has extensive experience in DNA typing services dedicated to helping researchers perform SNP typing studies of plant and animal genomes. Our PCR-RFLP and PCR-SSCP based methods are cost-effective, with high experimental turnaround, and our services provide researchers with strategies to screen for mutants in plants and animals.

PCR-RFLP

PCR-RFLP is the most classical method for SNP typing. When the target material is amplified with specifically designed PCR primers, the corresponding PCR amplified fragment often needs to be enzymatically cleaved to detect its polymorphism because the number of base mutations, insertions or deletions at the specific site is so small that no polymorphism occurs. For SNP loci that do not have a suitable enzymatic cut site, all SNP loci can be analysed by RFLP by changing the individual bases at the 3' end of the PCR primer to introduce a restriction endonuclease cut site, making the site a common enzymatic cut site.

Technical features

• Easy to use, no special equipment required.
• Low cost, suitable for medium volume samples, but also suitable for projects with high accuracy requirements or small sample sizes (a few or dozens).
• The test can distinguish between pure and heterozygous genotypes.
• Reliable and reproducible results.

Our services can be applied to the following research areas

• Genetic mapping
• Understanding the genetic structure and genetic relationships of varieties
• Identifying and screening for superior genetic variation

PCR-SSCP

PCR-SSCP is an analytical technique for detecting point mutations based on conformational differences in DNA. In its natural state, the sequence of single-stranded DNA bases determines its specific spatial conformation. If the sequence of the amplified target DNA fragment determines its specific spatial conformation. If a base variation occurs in the sequence of an amplified target DNA fragment, it may cause a change in the spatial conformation of its single strand, thus showing the specificity between the DNA of different individual organisms by their different migration rates during single strand electrophoresis.

Technical features

• Easy to use
• Fast method
• Sensitive detection

Our services can be applied to the following research areas

• Identification of gene mutations
• Identification and characterisation of polymorphisms in multiple genes
• Analysis of genetic pathogenic genes
• Characterisation of alleles of linked genes

Instructions for providing samples

Service workflow

Fig 2. Services workflow

Our advantages and features

• Our experienced technical team and standardised procedures ensure that we provide accurate and reliable results.
• We provide technical support services to our clients, from providing analysis solutions to providing professional analysis reports, and we are available to answer any questions you may have about the progress of your project.
• With high quality and fast service, we ensure that the results are delivered to our clients in the shortest possible time.

CD Genomics has many years of experience in SNP typing services and is well recognised by clients and industry professionals alike, with an excellent reputation. If you are interested in us, please feel free to contact us, we look forward to working with you!