BSA is an analysis method that mixes individuals with extreme traits in a segregated population and constructs two pools. By analyzing the difference sequences between the two pools, it can rapidly locate molecular markers closely linked to the target gene. This method is widely used in plant and animal gene localisation and plays an important role in accelerating crop breeding improvement.
CD Genomics provides BSA services with cost-effective features, mainly in terms of small sequencing libraries, short experimental cycle time, accurate and reliable results, and more detailed analysis for candidate gene screening. We have successfully served species including but not limited to rice, maize, soybean, peach, sugarcane, kale, cabbage, sorghum, cucumber, watermelon, potato, tomato, and others, especially for economically important traits such as insect resistance, disease resistance and high yield.
BSA allows for finer mapping of closely linked candidate regions screened for larger segregating populations on the genome, directing fine annotation of functional genes to the region and obtaining trait-associated candidate genes. BSA has been widely used for dozens of traits in many species due to its rapid, accurate and cost-effective nature. The main applications are currently in the following research areas.
QTL-seq to locate quantitative trait loci.
Mutmap to locate mutagenic loci.
Initial gene targeting for quantitative traits with quality traits or master effect genes.
Single trait studies.
Instructions for providing samples
Temporary (e.g. F1 and F2 segregating populations) or permanent populations (e.g. recombinant selfing lines, near-isogenic lines, double haploid lines, backcross inbred lines, etc.) of a certain size, with significantly different parental material for traits and with no less than 10% polymorphism between parents.
Trait segregating populations with as many individuals per population as possible, preferably no less than 20 (single plants), the more material the better to remove background noise.
For species without a reference genome, it is advisable to construct BAC libraries of the parents to lay the foundation for downstream localisation studies of strongly linked markers. For more precise annotation of functional genes it is preferable to have expression profiles or EST sequences.
Sample concentration ≥ 30 ng/μL, total ≥ 6 μg , OD 260/280 = 1.8-2.0.
BSA technical routes
Our advantages and features
We have a larger mixing pool size.
We can perform genome-wide 10,000+ SNP scans on species quantitative genotype frequency statistics.
Third generation molecular marker SNPs.
Personalised analysis solutions.
CD Genomics has extensive experience in BSA projects and will recommend the appropriate analysis protocol for a particular population and trait, helping clients to select different analysis strategies depending on the size and complexity of the species genome and the purpose of the study. We have a full quality control service to ensure reliable results, and we offer reference genome alignment, SNP and InDel detection and annotation, association analysis and linked region annotation. To date, we have successfully completed the localisation of nearly thousands of traits in hundreds of species. If you are interested, please feel free to contact us.
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OUR MISSION
CD Genomics is propelling the future of agriculture by employing cutting-edge sequencing and genotyping technologies to predict and enhance multiple complex polygenic traits within breeding populations.
