Reduced-Representation Genome Sequencing (RRGS) is the sequencing of a portion of the genome. It is a technique that uses restriction endonucleases to enzymatically cleave genomic DNA and perform high-throughput sequencing of enzyme-segmented segments. The simplified genome technology can greatly reduce the complexity of the genome because only the enzyme slice segment is sequenced, and it is not limited by the reference genome, and species without reference genome can also use this technology for molecular marker identification and development. Commonly used RRGS include RAD and GBS.

CD Genomics has extensive experience and years of expertise in RRGS for plants and animals, and we are committed to providing higher quality simplified genome sequencing for plants and animals to our customers worldwide. We have a simpler and faster library construction process and a variety of advanced sequencing platforms to help our clients achieve efficient genome-wide genetic and epigenetic variation in parallel. Up to now, we have worked on projects covering more than 20 species of plants and animals, helping to achieve breakthroughs in gene mining for important target traits and census of core plant and animal resources.

Technical features of RRGS for plants and animals

• Cost-effective. Without the high cost of whole-genome sequencing, and covering as much genome-wide variation information as possible, suitable for large sample size studies.
• Wide applicability. With or without reference genes, diploid or polyploid.
• High accuracy. With an average coverage depth of at least 10X for each digest tag, ensuring high accuracy of molecular markers.
• Fast and efficient. With the shortest time to complete the study of large sample size.

The RRGS services we offer can be used for the following studies

• Molecular marker development
• Molecular breeding
• Genetic mapping
• Gene/QTL localization
• Population genetic analysis
• Germplasm identification
• Plant and animal core resources census
• Target trait gene mining

Instructions for providing samples

RRGS workflow

Fig 2. RRGS workflow

Our advantages and features

• Advanced equipment. We have the most advanced sequencing platform.
• Technologically advanced. Our experienced experts use the most advanced technology to provide the highest quality service to our clients.
• Standardized service process. We have strict standards for our services to ensure that we deliver the most reliable experimental results for our clients.
• Customized experimental protocols. Our technical team is flexible to customize experimental protocols according to our clients' experimental needs.

CD Genomics provides RRGS services, which have been widely used in plant and animal research, helping researchers to obtain a large amount of genomic variation information at low cost, providing an important technical means to carry out population diversity and genetic structure analysis as well as population evolution and adaptive mechanism analysis, if you are interested in us, please feel free to contact us.