Small RNA Sequencing

Small RNA Sequencing

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Small RNA sequencing

Small RNA, as important functional molecules in living organisms, mainly include miRNA, ncRNA, siRNA, snoRNA, piRNA, rasiRNA and so on. They induce gene silencing through various sequence-specific gene silencing effects, such as RNA interference, translation inhibition, heterochromatin formation. Thus regulating various cellular physiological processes such as cell growth and development, stress response. Using the second generation of high-throughput sequencing technology, we can obtain a large amount of small RNA sequence information. Small RNA sequencing is widely used in life sciences, helping researchers to effectively identify known small RNA and discover new small RNA and their target genes.

CD Genomics has an advanced sequencing platform that accepts multiple sample types for small RNA sequencing and provides reliable and accurate data results. Based on high quality sequencing of small RNA regulating genes at transcriptional and post-transcriptional stages, our small RNA sequencing services are now widely used in plant and animal research and are dedicated to help researchers discover the important roles of small RNA in important plant and animal life processes.

In plant research. We help our clients to further reveal new regulatory mechanisms of miRNAs in plants, explore and discover new sRNA taxa, and provide valuable reference basis for their subsequent research. Small RNA sequencing has important applications in the study of biological processes such as plant growth and development, stress response, and evolutionary adaptation.

In animal research. Through small RNA sequencing, we have found that small RNA play important roles in individual animal life activities such as growth, development,differentiation, hormone secretion.We have found some non-coding RNA that regulates economically important traits in animals, such as dairy cows, pigs, which is important for these economically important traits.

Our small RNA sequencing can be applied to the following studies

  • The differentiation and development of plant and animal tissues and organs.
  • Research on symbiosis of plant and animal fungi.
  • Characterization of prokaryotic bacteria.
  • Pathogenic regulatory mechanism of plant and animal.

Advantages of small RNA sequencing technology

  • Study small RNA molecules directly from the nucleotide level.
  • High throughput analysis can be performed for any species.
  • High sensitivity and high coverage, with the ability to detect rare transcripts with very low abundance.
  • The raw data generated by sequencing is compatible with a variety of analysis software.

Instructions for providing samples

Sample type RNA
Concentration 200 ng / μL
Total RNA > 0.3 %
RIN > 8.0
Quantity 5000 ng
OD 260/280 ratio of 1.8 to 2.2
Storage and transportation Stored at -20 °C, shipped on dry ice
Others Please note the sample name, concentration, volume, preparation time, solvent name and species origin

Small RNA sequencing workflow

Small RNA sequencing workflow- CD GenomicsFig 2. Small RNA sequencing workflow

Features of our services

  • Real-time project progress tracking to keep clients up-to-date with project progress.
  • Advanced sequencing platform and years of project experience to deliver the most accurate and reliable data results for you.
  • Efficient resolution of project feedback to improve service efficiency.
  • Comprehensive after-sales service to solve your worries.

CD Genomics' small RNA sequencing services are well recognized by our customers. If you are interested in us, please feel free to contact us.

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CD Genomics is propelling the future of agriculture by employing cutting-edge sequencing and genotyping technologies to predict and enhance multiple complex polygenic traits within breeding populations.

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