Microsatellite (SSR) sequences are polymorphic DNA markers consisting of nucleotide sequences of 1-6 bases in length, repeated in tandem. SSR DNA is a new type of highly polymorphic DNA genetic marker system developed rapidly in recent years, which is widely present in all kinds of eukaryotic genomes, and it has more characteristics such as multiple species, wide distribution, high polymorphism, high heterozygosity, low recombination rate, and reliability. Therefore, it is a very ideal molecular marker.

CD Genomics is equipped with an advanced sequencing platform developed for SSR analysis. Our SSR analysis services require less sample volume and shorter experimental cycle time, enabling faster, simpler and more accurate SSR analysis. We have currently completed SSR analysis for livestock and fish. In addition, our SSR analysis services are widely used to determine natural variation based on genotypes of organisms, further contributing to research in the field of plant and animal population genetics and agriculture.

Main process

• Design experimental protocols by searching databases or articles. Including the  selection of fluorescent markers, primer design, synthesis and labeling, selection of internal standards, etc.
• PCR amplification.
• Complete electrophoresis scan on sequencer.
• Data analysis and processing. The collected data are processed and analyzed to provide information on the size and number of product fragments.

Fig1. SSR analysis workflow.

Client sample required information

SSR analysis involves PCR amplification using fluorescently labeled primers or genomic DNA, followed by allele analysis by capillary electrophoresis (CE) or electrophoretic separation of labeled PCR products by size. Therefore, customers need to differentiate samples according to the situation of the samples.

SSR analysis services can be used in the following studies

• Genetic map construction
• Marker-assisted selection
• Genetic testing
• Establishing DNA fingerprints
• Quantitative trait loci localization
• Genetic diversity assessment
• Variety identification
• Accelerated breeding

Service features and advantages

• Accuracy. We have the most advanced sequencer testing platform to provide you with accurate experimental data. In addition, your data will be professionally analyzed and corrected by us.
• Professional. Professional technical support and senior project management, to solve any technical and service questions for you.
• High efficiency and faster. PCR samples amplified by fluorescent primers, and deliver the results for you in the shortest time.
• More timely and clear. Timely update of project information to ensure customers are informed of project progress.
• High cost performance. We have high quality services and appropriate rates to reduce your worries about the progress of your project.

CD Genomics provides customized SSR analysis services that can meet various needs of our clients. If you are interested in us, feel free to contact us. We can provide you with professional and comprehensive SSR service research. We have professional technical services, experienced experts, and one-stop comprehensive services, start your project with us!